- 期刊
生醫孔隙纖維表面在不同酸鹼值下固定肝素之研究
Study of Immobilized Heparin in Varied pH Values onto Porous Non-Woven Fabric Surface
摘要
本研究以陣列式低溫氧氣微波電漿對聚丙烯不織布表面進行活化接枝丙烯酸單體，再將肝素（heparin）藉促進劑（coupling agent），於不同pH值環境下固定於其表面上，探討不同pH值對肝素固定的影響。經改質的材料以ATR-FTIR、ESCA來對改質基材進行表面定性分析；以Toluidine Blue 做處理後表面肝素固定量之定量分析；將改質後的試片與血漿接觸，量測培養後血漿的aPTT（部分活化凝血時間）、TT（凝血時間）的時間改變及fibrinogen（纖維蛋白）的濃度變化、同時也計算血小板在材料試片上的吸附率，來確認固定肝素表面的活性。結果顯示：在丙烯酸接枝量約為236μg.cm^(-2)下，肝素固定量受到pH 環境影響約在9-13μg.cm^(-2)之間；血液凝固測試結果則顯示：血漿經與肝素固定的試片培養後，血漿中的fibrinogen濃度及血小板吸附率隨環境pH值增加而略減，但aPTT則是隨pH值溶液環境的上升而較TT的時間顯著延長，這可證實經誘導固定的肝素聚丙烯纖維表面仍具生物活性，且會受到製備時之pH值的改變或不同離子強度環境而影響其固定量、同時影響生物活性程度。
並列摘要
This study utilized antenna-coupling O2 microwave plasma to activate non-woven polypropylene (PP) fabric surface and graft with acrylic acid (AAc). Heparin was then immobilized using a carbodiimide as coupling agent. Surface analyses by ATR-FTIR and ESCA traced the presence of the functional groups. To quantify the immobilized heparin, Toluidine Blue method was used. The blood clotting effect of heparin-bonded surfaces was evaluated by activated partial thrombin time (aPTT), thrombin time (TT), fibrinogen concentration and platelet adhesion on the porous surfaces. Current result demonstrated that PP surface with AAc-grafted amount of ca. 236μ g.cm^(-2); the immobilized heparin was gained ca. 9-13μ g.cm^(-2), varied with pH values during preparation. After human plasma incubated with heparinized surfaces, the concentration of fibrinogen and the ratio of platelets adhesion were decreased in human plasma; however, the required time for thrombination (by aPTT and TT tests) was significantly increased. Consequently, the bioactivity on the heparinized surfaces was validated; and this was influenced by the variation of pH values in sample preparation or thus ionic strength formed at the modified surfaces. The immobilized amount was simultaneously correlated with the degree of bioactivity.