Development and Electrical Analysis of DNA Aqueous Solution on Microchannel Biochip

用於DNA水溶液電性分析之微通道生物晶片研發

潘至理(Chih-Li Pan) ； 姜彥安(Yen-An Chiang) ； 管傑雄(Chieh-Hsiung Kuan) ； 郭敏玲(Ming-Ling Kuo) ； 詹國禎(Gwo-Jen Jan)

《Journal of Medical and Biological Engineering》 22卷4期 (2002/12) Pp. 161-169

摘要

本研究利用微機電技術來設計並製作一種以矽為基板的微流道生物晶片。實驗中將醋酸離子水溶液和或去氧核糖核酸分子水溶液放置於微流道中當作實驗試劑，當外加電場於微流道中時，來了解並分析樣品分子的直流電特性。此外，再利用高分辨度的量測系統來精確地量測此時實驗試劑對應於不同外加電壓所產生的電流值曲線。在本實驗中，成功地區分出dATP，dGTP，dTTP，以及dCTP不同的實驗試劑。由於堆積在電極的電荷產生擴散效應來抵銷外加電場所產生的電場效應，所量測到的電流對時間的曲線表現了震盪現象。本實驗初步的研究成果證明了本研究所設計的生物晶片和量測方法能用於區分不同濃度的醋酸水溶液，以及相同濃度但不同種類的去氧核糖核酸分子，這種量測方法應該有助於生物醫學診斷科技的研究以及應用。

關鍵字

生物晶片 ； 微通道生物晶片 ； 去氧核糖核酸分析 ； 電性量測

並列摘要

A microchannel biochip employed by micro-electro-mechanical systems (MEMS) and based on Si substrate was designed and developed in the research. The ionic CH3COOH or DNA aqueous solutions were filled in the microchannel for the reagents in the experiment and the DC electrical characterization of the samples was carried out and analyzed when applying voltage in the microchannel. Furthermore, the curve of current versus voltage of these reagents was measured precisely by a high-resolution measurement system. In the experiments, it was successfully to distinguish the reagents as dATP, dGTP, dTTP, and dCTP. Moreover, the curves displayed oscillation phenomena with time because the accumulating charges generated a diffusion effect to counteract the electrical drift effect caused by applying voltage. The initial results demonstrated that the designed microchannel biochip is feasible to be used and distinguish different DNA molecules. The method should be beneficial for the advanced studies and applications of biomedical diagnostic technology.

並列關鍵字

Biochip ； Microchannel biochip ； DNA analysis ； Electrical characterization