The light distribution and light path in a biological tissue is determined by its optical properties. In most applications of biomedical optics, the intensity of light source has to be well controlled to achieve the best effect and to prevent possible harm. The key factors for making decision are the optical properties. The purpose of this study is to build a spectral image measurement system to capture the diffusive reflectance image when monochromatic light is projected on the surface of a scattering media. A Monte Carlo model is also used to simulate the image of diffusive reflectance. The effect of absorption and scattering coefficients on the diffusive reflectance is studied by modifying their values in model. The reflectance intensity at the center position and the slope of radial intensity profile are features that can be used to evaluate the optical coefficients.