- 期刊
Investigation of Neurochemical Techniques for Assessment of Nerve Regeneration within Polymer Guides
並列摘要
The use of proteins associated with neurite outgrowth, synapse formation, and Schwann cell proliferation as surrogate measures for peripheral nerve regeneration is evaluated. Rat sciatic nerve regeneration across a 10-mm gap in silicone rubber conduits (SRCs), genipin-crosslinked gelatin conduits (GGCs), and porous GGCs (PGGCs) is observed. The axonal growth of regenerated nerves after 8 weeks is determined using light microscopy and computer-based quantitative image analysis. The expressions of axonal growth-associated protein 43 (GAP-43), synaptic protein synapsin I, and transforming growth factor type-β (TGF-β) in regenerated nerves are assessed using Western blot analysis. The nerve regenerates in the SRCs has a larger area and more myelinated axons compared to those in the PGGCs and the GGCs. The levels of GA P-43 and synapsin I, but not TGF-[l. are well correlated with axonal growth in the regenerated nerves. These data suggest that SRCs provide a more stable and suitable growth environment for regenerating axons than do PGGCs and GGCs. In addition, GAP-43 and synapsin I may be useful indicators of the status of regenerating axons in bridging conduits.
延伸閱讀
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