Immune Inhibitory Factor in Mammalian Tissues

哺乳動物組織中的免疫抑制因子

五一世叡(Soo-Ray Wang)

《The Changhua Journal of Medicine》 6卷4期 (2001/10) Pp. 213-218

摘要

從哺乳類生物肝臟分離出一種蛋白質，可以抑制由細胞分裂素所引發的淋巴球增生，這種蛋白質稱為肝臟抑制蛋白(LIP)，我們由小鼠肝臟純化LIP，經由多層純化步驟，包括硫酸銨沈澱，DEAE色層分析，hydroxylapatite色層分析及G-150 superfine Sephadex膠質過濾法。所分離出來的LIP經證實是高純度的，其抑制淋巴球的活性增強了37倍。我們證明LIP即是精胺酸酶，它可以抑制T-及B-淋巴球的增生。經研究它的特性，它可以忍受溫度到65℃而不失抑制淋巴球的活性，而酸鹼度在3到11之間具有活性，分子量為105 kDa。在抑制淋巴球增生的胺基酸酶的篩選中，精胺酸酶名列活性第三，而三者之中，精胺酸酶是唯一源自哺乳類，所以人體的精胺酸酶將可適用於臨床，來治療多種免疫疾病，譬如器官移植的排斥反應、紅斑性狼瘡。

關鍵字

淋巴球增生 ； 精胺酸酶 ； 免疫抑制因子 ； 純化 ； 哺乳類 ； 肝臟抑制蛋白

並列摘要

A protein obtained from mammalian livers capable of inhibiting mitogen-induced lymphocyte proliferation, is called ”liver-derived inhibitory protein”, or LIP. LIP was purified from mouse liver homogenates by various purification procedures, including ammonium sulfate precipitation, DEAE chromatography, hydroxylapatite chromatography, and G-150 superfine Sephadex gel filtration. The LIP was proven to be very pure, and its inhibitory activity was improved 37-fold. The LIP was identified to be arginase. LIP can inhibit both T- and B-lymphocytes. It is temperature stable up to 65℃ and pH stable from 3 to 11. LIP molecule weight is 105 kDa. Arginase is the only mammalian amino acid-degradation enzyme that can potently inhibit lymphocyte proliferation. Therefore, arginase of human origin has a great potential of therapeutical benefit in treating immunologic diseases, such as transplant rejection, systemic lupus erythematosus, etc.

並列關鍵字

lymphocyte proliferation ； arginase ； immune inhibitory factor ； purification ； mammalia ； liver derived inhibitory protein