Alterations of Plasma thiol-related Antioxidants and Mitochondrial DNA Mutation in Hair Follicles of Smokers

吸煙族群中血漿甘硫類抗氧化劑與毛囊粒線體基因斷裂發生率之相關分析

劉青山(Chin-San Liu) ； 陳逸芸(Yi-Yun Chen) ； 蔡靜姍(Ching-Shan Tsai) ； 郭珍菱(Chen-Ling Kuo) ； 魏耀揮(Yau-Huei Wei)

《The Changhua Journal of Medicine》 7卷4期 (2002/10) Pp. 226-233

摘要

背景及目的：本研究主要是探討長期吸煙族群中血漿甘硫類抗氧化劑(thiol-related antioxidants)對毛囊粒線體基因4,977鹸基對斷裂保護之相關分析。
方法：我們的收集來自彰化基督教醫院共選擇40名健康非吸煙者與22名吸菸且吸煙指標大於5者，並檢測血漿甘硫類抗氧化劑(thiol-related antioxidants)與毛囊粒線體基因4,977鹸基對斷裂。
結果：吸煙族群較非吸煙族群有較低的之麩胱甘硫轉移酵素(glutathione S-transferase)、麩胱甘硫氧化酵素(glutathione peroxidase)活性、與較高頻率之粒線體基因4，977鹸基對斷裂，然而發生粒線體基因4，977鹸基對斷裂之頻率與年齡成正比，而與血漿麩胱甘硫轉移酵素活性反比，同時發現如果血漿麩胱甘硫轉移酵素活性低於5.66 U/I之吸煙者，其發生粒線體4，977鹸基對斷裂之發生率較血漿麩胱甘硫轉移酵素活性高於5.66 U/I之吸煙者多出7.2倍(OR=7.2, P=0.020)。
結論：吸煙族群中較高麩胱甘硫轉移酵素活性可能扮演細胞粒線體基因穩定之角色。

關鍵字

粒線體基因斷裂 ； 甘硫類抗氧化劑 ； 麩胱甘硫轉移酵素 ； 吸煙

並列摘要

Background and purpose: The effects of long-term smoking on mitochondrial DNA (mtDNA) deletions in hair follicles were investigated in subjects with different thiol-related antioxidant capacities.
Methods: Twenty-two male smokers with a smoking index of greater than 5 pack-years and without any known systemic diseases were recruited for this study. Forty healthy males without smoking history were also included as control subjects. Thio-related antioxidants in plasma and a 4,977-bp segment of deleted mtDNA (dmtDNA) in the hair follicle were tested in all subjects.
Results: The activities of glutathione S-transferase (GST) and glutathione peroxidase in blood plasma were significantly decreased in smokers. The levels of glutathione and protein thiols in whole blood and the incidence of a 4,977-bp dmtDNA were significantly increased in smokers. A significantly higher incidence of the 4,977-bp dmtDNA was found in smokers with plasma GST activity less than 5.66 U/L (OR=7.2, P=.020). By using a multi-covariate analysis of variance (ANOVA) and logistic regression, we found that age and low plasma GST activity were the only two risk factors causing the 4,977-bp dmtDNA.
Conclusion: These results suggest that smoking depletes antioxidants and causes mtDNA deletions and that plasma GST may play an important role in the preservation of mitochondrial genome in the somatic cells of smokers.

並列關鍵字

mitochondrial DNA deletion ； plasma thiol-related antioxidant ； smoking ； glutathione S-transferase