Peripheral Blood Monocytes Acquire the Morphology and Phenotypes of Antigen-Presenting Dendritic Cells When Cultured in Granulocyte-Macrophage Colony Stimulating Factor in Combination with Interleukin

背景及目的：樹突狀細胞為抗原呈獻細胞在主要免疫反應扮演重要角色，樹突狀細胞能夠專一性的將抗原吞噬、處理並呈獻給T細胞，而將之活化。本實驗研究目的是比較體外培養出的樹突狀細胞以介白質素-4或干擾素-α合併血球生長激素(GM-CSF)刺激。方法：自10位正常人周邊血液分離出單核球細胞，以介白質素-4或干擾素-α合併血球生長激素GM-CSF培養，經過7天的培養後，利用流式細胞儀來分析。結果：以介白質素-4或干擾素-α合併血球生長激素GM-CSF刺激培養的周邊血液單核球細胞表現出樹突狀細胞的型態與特性，經過細胞激素的影響，這些細胞明顯增加CD86（B7-2輔助刺激因子）、主要組織相容性複體MHC class I(HLA-A,B,C)和CD11c的表現。結論：許多研究發現樹突狀細胞對於癌細胞或癌蛋白可以產生具有對抗腫瘤免疫反應。由於樹突狀細胞非常稀少，若能有效地從周邊血液中分離出單核球細胞並體外大量培養，可幫助更加了解樹突狀細胞的特質並幫助我們評估是否可強化針對一般低免疫性表現癌症細胞之免疫治療。

關鍵字

樹突狀細胞；干擾素-α ；介白質素-4

並列摘要

Background and purpose: Dendritic cells (DCs), highly potent antigen presenting cells that play a center role in generating primary immune responses, are specialized for antigen uptake, processing, and presentation to T cells. They are distinguished for the extraordinary ability to prime naive T cells. We investigated and compared the effects of granulocyte-macrophage colony stimulating factor (GM-CSF) in combination with interleukin-4 (IL-4) or interferon α (IFN-α) in promoting DC differentiation from peripheral blood monocytes in vitro. Methods: Peripheral blood monocytes were isolated from 10 normal human donors and cultured with IL-4 or IFN-α in combination with GM-CSF for 7 days, labeled with fluorochrome-conjugated monoclonal antibodies and analyzed using flow cytometry. Results: Cell cultures with IL-4 or IFN-α in combination with GM-CSF developed morphology and phenotype of DCs. High levels of CD86, HLA-A, B, C, and CD11c were observed in these DCs that are generated from monocytes under the influence of exogenous cytokines. Conclusion: DCs pulsed with tumor lysates or peptides generate potent antitumor immune responses. Methods to produce these rare cells in vitro are valuable because they would enable investigators to study their biological properties. The ability to generate large numbers of DCs from peripheral blood mononuclear cells ex vivo and load them with tumor-specific antigens will enable researchers to evaluate the feasibility and efficacy of inducing immunity against poorly immunogenic tumors, as in most human malignancies.

並列關鍵字

dendritic cells ； interferon-α ； interleukin-4

國際替代計量

Peripheral Blood Monocytes Acquire the Morphology and Phenotypes of Antigen-Presenting Dendritic Cells When Cultured in Granulocyte-Macrophage Colony Stimulating Factor in Combination with Interleukin

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