Preliminary Results of Expression Spectrum of Matrix Metalloproteinases in Non-small Cell Lung Cancer

Background and purpose: Previously, we have detected overexpression of dihydrodiol dehydrogenase (DDH), and enzyme closely associated with drug resistance and hence with poor prognosis in patients with non-small cell lung cancer (NSCLC). Presently, we probed the gene expression of matrix metalloproteinases (MMPs) of normal and lung cancer specimens. Methods: A combination of polymerase chain reaction (PCR)-select cDNA subtractive hybridization, microarray, differential display and reverse transcriptase (RT)-PCR to identify differential mRNA expression spectra of MMPs. Results: Three panels of gene expressions were detected. The expressions of MMP-1, -7 and -10 increased dramati-cally in the tumor fraction of NSCLC. Lung adenocarcinoma cells also overexpressed these MMP species in vitro. Conclusion: NSCLC cells have a different MMP expression profile than normal lung cells. This distinct profile could be useful in identifying such cells, and in as a reference for NSCLC patients scheduled to receive a class-specific MMP inhibitor in adjuvant therapy.

並列關鍵字

matrix metalloproteinases ； non-small cell lung cancer ； gene expression

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