- 期刊
The Effect of Dehydroepiandrosterone Pretreatment on Pertussis Toxoid-Induced Immune Response in BALB/C Mice
預告施用氫表友雄固李玉酮對百日咳類毒素誘發BALB/c老鼠免疫反應的影響
並列摘要
Background and purpose: Dehydroepiandrosterone (DHEA), one of the predominant androgens secreted by the adrenal cortex, is a potential immunoregulator. In this report, the modulatory effects of DHEA on pertussis toxoid (PT)-induced antibody response, total immunoglobulin production and cytokine secretion were investigated. Methods: Young mice (8-12 wks) were orally administrated with DHEA for 7 consecutive days, followed by immunization with formaldehyde-inactivated PT. The antibody activity against PT in mouse sera was titrated and the serum levels of immunoglobulins (IgA, IgG, IgM) measured. Spleen cells were isolated after animals were sacrificed and cultured in vitro in the presence of lipopolysaccharide or Con A. The viable cell numbers were estimated, and the concentrations of immunoglobulins and cytokines (interleukin-2, interferon-g, interleukin-4) in culture supematants were measured. Results: Oral administration of DHEA prior to PT immunization significantly inhibited IgG, IgA and IgM secretion, and suppressed both Th1-type and Th2-type cytokines secretion by spleen cells. DHEA down-regulated the spleen cell functions, which resulted in a significant reduction in PT-induced antibody response. Conclusion: Data in this report suggested that oral administration of DHEA prior to PT immunization suppressed anti-PT antibody response and spleen cell functions.
延伸閱讀
- Wu, G. H., Chang, H. C., Liou, C. J., & Tseng, J. (2003). 去氫表雄固酮對幼鼠免疫注射百日咳類毒素的佐劑效應. The Changhua Journal of Medicine, 8(4), 233-244. https://www.airitilibrary.com/Article/Detail?DocID=16801504-200310-8-4-233-244-a
- Yang, C., Chen, B. R., Hou, C. H., Cheng, Y. L., Shieh, J. P., Ho, S. T., & Wang, J. J. (2005). 一個含Ketorolac Tert-Butyl酯之藥物貯存劑型在大白鼠測試有長效之抗發炎及止痛作用. 疼痛醫學雜誌, 15(1), 1-8. https://doi.org/10.29792/TTJP.200503.0001
- 鄭詠仁（2008）。KMUP-1反轉野百合鹼誘導的肺高壓大鼠肺動脈之鉀離子通道及Rho-Kinase的功能不良〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0011-0907200804350700
- 吳建志（2007）。使用體外膜氧合術(ECMO)的成人其萬古黴素(vancomycin)藥品動態學及劑量學研究〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2007.02999
- Tzeng, J. I., Chu, C. C., Feng, P. H., Hou, C. H., Shieh, J. P., Ho, S. T., & Wang, J. J. (2005). 一個含非類固醇消炎劑之長效藥物貯存劑型－Ketorolac苯酯在大白鼠之止痛與抗發炎測試. Acta Anaesthesiologica Taiwanica, 43(1), 17-22. https://www.airitilibrary.com/Article/Detail?DocID=02541319-200503-43-1-17-22-a