B Group Vitamin and Methylenetetrahydrofolate Reductase C677T Related Alteration of Plasma Homocysteine

血漿中同半胱胺酸與維生素B群及甲基四氫葉酸還原酶基因多型性之相關分析

巫錫霖(Shey-Lin Wu) ； 杜思德(Shih-Te Tu) ； 鄭文玲(Wen-Ling Cheng) ； 李東杰(Dong-Jay Lee) ； 陳暉雯(Haw-Wen Chen) ； 江惠琴(Hui-Chin Chiang) ； 郭珍菱(Ching-Ling Kuo) ； 黃靜珊(Ching-Shan Huang) ； 劉青山(Chin-San Liu)

《The Changhua Journal of Medicine》 10卷3期 (2005/07) Pp. 124-129

並列摘要

Background and purpose: Hyperhomocysteinemia was proved as one of risk factor involved in the pathogenesis of atherosclerosis, and methylenetetrahydrofolate reductase (MTHFR) 677TT genotype have been identified as a potential genetic factor involved in the hyperhomocysteinemia. In this study, we made a hypothesis that levels of B group vitamin (Vit B) and age status may modify the impact of MTHFR 677TT genotype-associated hyperhomocysteinemia. Methods: A total of 541 subjects without dyslipidemia, alcoholism, diabetes, hypertension and cardiovascular disease were recruited. The plasma total homocysteine (Hcy) concentration was determined by High Performance Liquid Chromatography (HPLC). The plasma Vit B6 concentration was determined using a Vit B6 reagent kit. Plasma folate and Vit B12 were analyzed using radioimmunoassay (RIA). MTHFR C677T polymorphism was identified by Polymerase Chain Reaction (PCR) method.
Results: MTHFR 677TT carriers had higher plasma levels of Hcy compared with subjects harboring CC/CT genotypes (P ＜ 0.05). Moreover, the evidence of MTHFR 677TT genotype-related hyperhomocysteinemia was only inspected in the MTHFR 677TT carriers with age greater than 50 and combined with lower level of folate (＜ 8.3 ng/mL), Vit B12 (＜ 618 pg/mL), Vit B6 (＜ 36 μg/L).
Conclusions: We concluded that plasma B group Vit and age status may be considered as two arbitration factors involved in the pathogenesis of MTHFR 677TT genotype-related hyperhomocysteinemia.

並列關鍵字

MTHFR ； homocysteine ； B group vitamin