Albendazole and Long-Dan-Cao Co-therapy against Angiostrongylus cantonensisinduced Matrix Metalloproteinase-9 and Eosinophilic Meningitis

評估Albendazole及龍胆草混合治療對鼷鼠感染廣東住血線蟲誘發基質金屬蛋白叠－9及嗜伊紅性腦膜炎之作用

陳科銘(Ke-Min Chen) ； 王啟屏(Chi-Pin Wang) ； 賴世展(Shih-Chan Lai)

《中山醫學雜誌》 17卷1期 (2006/06) Pp. 63-70

https://doi.org/10.30096/CSMJ.200606.0007

摘要

抗蠕蟲葯albendazole能殺死感染腦部的廣東住血線蟲(Angiostrongylus cantonensis)幼蟲，但是被殺死的蟲體死時會引起腦部傷害的免疫反應。龍草(Long-Dan-Cao)是一種很普通的中國中草藥。為了分析albendazole及龍草混合治療鼷鼠感染廣東住血線蟲誘發蛋白分解酵素(proteolytic enzymes)及嗜伊紅性腦膜炎(eosinophilic meningitis)之作用，本試驗分析療效的指標性因子包括：1)組織學的改變；2)基質金屬蛋白叠-9(matrix metalloproteinase-9, MMP-9)；3)白蛋白(albumin)；及4)嗜伊紅性白血球(eosinophil)計數。這些結果顯示albendazole及龍胆草混合治療明顯抑制廣東住血線蟲所誘發之蛋白分解酵素，這些結果顯示以龍草輔助albendazole治療是相當有效的策略。

關鍵字

廣東住血線蟲 ； 嗜伊紅性腦膜炎 ； 基質金屬蛋白叠－9 ； albendazole ； 龍胆草

並列摘要

The anthelmintic agent albendazole can kill the Angiostrongylus cantonensis larvae that infect the brain in patients with eosinophilic meningitis or meningoencephalitis, but the dead larvae are capable of evoking a severe, brain damaging immune response. This prompted us to investigate the effect of using Long-Dan-Cao, one of the most wellknown Chinese herbal medicines. We, therefore, used A. cantonensis to induce proteolytic enzymes and eosinophilic meningitis in BALB/c mice in order to evaluate the effect of Long-Dan-Cao as well as the effect of using it as a co-therapy with albendazole by assay. Assay indicators for the therapeutic effect include: histological changes, matrix metalloproteinase-9, albumin, and eosinophil counts. Our results showed that the albendazole and Long-Dan-Cao co-therapy is effective against A. cantonensis-induced proteolytic enzymes and that Long-Dan-Cao has therapeutic value for parasitic meningitis.

並列關鍵字

Angiostrongylus cantonensis ； eosinophilic meningitis ； matrixmetalloproteinase-9 ； albendazole ； Long-Dan-Cao