人工光源對黃緣螢幼蟲基因表現之影響

The Impact of Artificial Light on Aquatica ficta Larvae Transcriptome

陳韻如(Yun-Ru Chen) ； 曾德維(David, Tzeng) ； 姜安蓉(Avalon Celeste Stevahn Owens) ； 吳加雄(Chia-Hsiung Wu) ； 蕭忠義(Chung-Yi Hsiao) ； 唐欣潔(Hsin-Chieh Tang) ； 鍾思林(Silin Zhong) ； 楊恩誠(En-Cheng Yang)

《台灣昆蟲》 38卷2期 (2018/06) Pp. 63-72

https://doi.org/10.6662/TESFE.2018007

摘要

近年來人工光源對螢火蟲族群維持帶來的衝擊與影響引起重視，雖有研究指出夜間人工照明會中斷螢火蟲交尾行為，但人工光源對螢火蟲幼蟲是否會造成影響仍待探討。本研究利用次世代定序技術，從分子層次探討黃緣螢(Aquatica ficta Olivier)幼蟲夜間暴露在人工光源下的基因表現。定序總共生成約532.8M RNA-seq reads，組裝出208,672條轉錄子，其中60,410條轉錄子完成功能註釋。差異性表現基因分析結果顯示，在夜間光照處理14天後，檢測到1,450個差異性表現基因，其中58條轉錄子表現被啟動／表現量上升，1,392條轉錄子停止表現／表現量下降。我們進一步分析差異性表現基因的生理功能分析，發現表現被啟動／表現量上升的轉錄子功能群與「內分泌調節」、「青春激素代謝」、「氧化還原途徑」等相關；而停止表現／表現量下降的轉錄子功能則與「生物合成核糖核蛋白複合物」、「初級代謝途徑大分子代謝途徑」、「細胞生物合成途徑」、「有機物質生物合成途徑」以及「滲透壓反應」相關。目前證據顯示短期夜間光照對螢火蟲幼蟲基因表現確實造成影響，但光照後對於螢火蟲幼蟲發育是否造成長期的影響仍待更多證據釐清。

關鍵字

黃緣螢 ； 夜間照明 ； 轉錄組 ； 差異性表現基因

並列摘要

Artificial light at night (ALAN) is considered one of the factors causing a decline in the firefly population. Although several studies have evaluated mechanisms through which ALAN interferes with the courtship of fireflies, the effect of ALAN on firefly larvae remains unknown. To investigate the effect of light pollution on firefly larvae from the molecular perspective, we used the next-generation sequencing method to profile the transcriptome of the larvae of yellow-rimmed firefly, Aquatica ficta Olivier, under artificial light treatment during the night time. A total of 532.8 million RNA-seq reads were generated for the transcriptome assembly from the firefly larvae exposed to artificial light as well as from those exposed to a regular light-dark cycle. Approximately 208,672 transcripts (unigenes) were obtained, and 60,410 unigenes were annotated. Compared with the control, we identified 1,450 differentially expressed genes (DEGs) from the larvae that underwent ALAN treatment during the dark cycle. From the 1,450 DEGs, 58 and 1,392 unigenes were found to be activated/upregulated and inactivated/downregulated, respectively. Subsequently, we performed gene ontology analysis and found that activated/upregulated unigenes are enriched in terms related to hormone level regulation, juvenile hormone metabolism, and oxidation-reduction process, whereas inactivated/downregulated unigenes are enriched in terms related to ribonucleoprotein complex biogenesis, primary metabolic process, macromolecule metabolic process, cellular biosynthetic process, organic substance biosynthetic process, and response to osmotic stress. Additional evidence is still required to demonstrate the long-term effects of ALAN on the transcriptome profile and development of fireflies.

並列關鍵字

Aquatica ficta ； artificial light at night ； transcriptome ； Differentially Expressed Genes