Introduction: Long-term exercise is associated with an important of immune function. All of the immune cells, natural killer (NK) cells, neutrophils, and monocytes (of the innate immune system) appear to be most responsive to the effects of acute exercise, both in terms of numbers and function. In response to long-term exercise, the only finding to date reported with some congruity between investigators is a significant elevation in NK cell activity. Objective: To assess the inhibition and recovery of primary cellular immunity in long-term exercise, 42 Km marathon race. Methods: PBMC (1×10^6) from 8 healthy marathon runner were incubated with monoclonal antibodies against CD16 for 20 min at RT. Cells were washed and then incubated with FITC-conjugated (Fab')2 goat' anti-mouse IgG for 20 min. They were thoroughly washed and resuspended with fluorescent assay buffer. Phagocytosis assay used heparinzed whole blood is incubated with the FITC-labelled E coli bacteria at 37℃ for 10 mins. The intensity of the positive surface immunofluorescence of 10^4 viable cells was measured with a FACS caliber flow cytometer. Results: In NK killing assay, the data was showed that NK cell cytotoxic activity was decreased significantly (p<.001) after long-duration exercise. In fifth day, however, the NK cell cytotoxic activity was recovered significantly (p<.001). The phagocytic activity before exercise was not different from post-exercise. The number of lymphocyte was decreased significantly (p<.001) after long-duration exercise. In addition, the percentage of NK cells in lymphocytes was decreased significantly (p<.001). Conclusion: In this study, we found not only the number of NK cells was decreased but the capability of NK cytotoxic was also decreased significantly after long-duration exercise, marathon. The ability of phagocytic cell was different particularly (p<.01) between preand post-long-duration exercise.
Introduction: Long-term exercise is associated with an important of immune function. All of the immune cells, natural killer (NK) cells, neutrophils, and monocytes (of the innate immune system) appear to be most responsive to the effects of acute exercise, both in terms of numbers and function. In response to long-term exercise, the only finding to date reported with some congruity between investigators is a significant elevation in NK cell activity. Objective: To assess the inhibition and recovery of primary cellular immunity in long-term exercise, 42 Km marathon race. Methods: PBMC (1×10^6) from 8 healthy marathon runner were incubated with monoclonal antibodies against CD16 for 20 min at RT. Cells were washed and then incubated with FITC-conjugated (Fab')2 goat' anti-mouse IgG for 20 min. They were thoroughly washed and resuspended with fluorescent assay buffer. Phagocytosis assay used heparinzed whole blood is incubated with the FITC-labelled E coli bacteria at 37℃ for 10 mins. The intensity of the positive surface immunofluorescence of 10^4 viable cells was measured with a FACS caliber flow cytometer. Results: In NK killing assay, the data was showed that NK cell cytotoxic activity was decreased significantly (p<.001) after long-duration exercise. In fifth day, however, the NK cell cytotoxic activity was recovered significantly (p<.001). The phagocytic activity before exercise was not different from post-exercise. The number of lymphocyte was decreased significantly (p<.001) after long-duration exercise. In addition, the percentage of NK cells in lymphocytes was decreased significantly (p<.001). Conclusion: In this study, we found not only the number of NK cells was decreased but the capability of NK cytotoxic was also decreased significantly after long-duration exercise, marathon. The ability of phagocytic cell was different particularly (p<.01) between preand post-long-duration exercise.