虹鱒（Oncorhynchus mykiss）吞噬細鮑對不同水生病原菌吞噬作用之比較

Comparison on Phagocytic Activity of Rainbow Trout (Oncorhynchus mykiss) Phagocytes Against Aquatic Bacterial Pathogens

徐榮彬(Jung-Pin Hsu) ； 余俊明(Jiunn-Ming Yu) ； 廖志明(Chih-Ming Liaw) ； 李維誠(Wei-Cheng Lee) ； 洪信雄(Hsin-Hsiung Hung) ； 劉正義(Cheng-I Liu) ； 簡茂盛(Maw-Sheng Chien)

《臺灣獸醫學雜誌》 28卷4期 (2002/12) Pp. 272-280

https://doi.org/10.7009/TVJ.200212.0272

摘要

The phagocytes in salmonid fish are believed to play the major role on their nonspecific immunity against most aquatic pathogens and irritants. In this trial, flow cytometric analysis was applied to detect and compare the phagocytic activity of rainbow trout (Oncorhynchus mykiss) phagocytes isolated from blood, head kidney and spleen, respectively. In general, the blood phagocytes showed higher capability of phagocytosis than spleen and head kidney phagocytes in engulfing Vibrio parahemolyticus, Vibrio anguillarum and Aeromonas hydrophilia. The results also demonstrated that bacteria opsonized with or without normal fish serum did not manipulate phagocytes to uptake and eradicate bacteria after 120 minutes incubation. However, the isolated phagocytes could enhance their phagocytic activity if increasing cultivation time from 2 to 24 hours. In addition, all phagocytes engulfed much more bacterial pathogen that could secrete hemolysin or their extracellular products (ECPs) could induce significant cytotoxicity on phagocytes.

關鍵字

吞噬細胞 ； 吞噬作用 ； 虹鱒 (Oncorhynchus mykiss)

並列摘要

The phagocytes in salmonid fish are believed to play the major role on their nonspecific immunity against most aquatic pathogens and irritants. In this trial, flow cytometric analysis was applied to detect and compare the phagocytic activity of rainbow trout (Oncorhynchus mykiss) phagocytes isolated from blood, head kidney and spleen, respectively. In general, the blood phagocytes showed higher capability of phagocytosis than spleen and head kidney phagocytes in engulfing Vibrio parahemolyticus, Vibrio anguillarum and Aeromonas hydrophilia. The results also demonstrated that bacteria opsonized with or without normal fish serum did not manipulate phagocytes to uptake and eradicate bacteria after 120 minutes incubation. However, the isolated phagocytes could enhance their phagocytic activity if increasing cultivation time from 2 to 24 hours. In addition, all phagocytes engulfed much more bacterial pathogen that could secrete hemolysin or their extracellular products (ECPs) could induce significant cytotoxicity on phagocytes.

並列關鍵字

Phagocytes ； Phagocytosis ； Rainbow trout ； Oncorhynchus mykiss