大腸桿菌與沙門氏菌廣泛傳布於人及動物,具多重抗藥性之人畜臨床分離株出現並受到重視。本試驗目的為檢測大腸桿菌與沙門氏菌之抗菌劑感受性與抗藥基因。自1997年至2001年由台灣豬隻臨床病例分離60株大腸桿菌與60株豬霍亂沙門氏菌,對常用抗菌劑進行最小抑菌濃度(MIC)試驗,並以聚合酶連鎖反應偵測毒力基因及抗藥基因。Amikacin及ceftiofur對大腸桿菌及豬霍亂沙門氏菌之MIC值最低;Cephalothin,doxycycline,florfenicol及gentamicin具中等抑菌能力;Flumequine對豬霍亂沙門氏菌具中等抑菌能力,但對大腸桿菌則無抑菌力;Ampicillin,chloramphenicol,chlortetracycline,nalidixic acid,kanamycin,streptomycin,sulfamethoxazole,tetracycline及trimethoprim對大腸桿菌及豬霍亂沙門氏菌均無抑菌能力。Integron於大腸桿菌及豬霍亂沙門氏菌之盛行率各為97%與95%;大腸桿菌的tem及floR抗藥基因盛行率各為38%和55%;豬霍亂沙門氏菌之tem,floR及spvC基因的盛行率分別為60%,25%與98%。由本實驗結果得知,豬隻分離之大部分大腸桿菌及豬霍亂沙門氏菌具多重抗藥性及Integron。為減少抗藥菌株發生必須建立用藥監測系統。
Escherichia coli and Salmonellae infection are very common in people and animals worldwide. The emergence of multidrug resistance in these bacteria is increasing significantly in both animal industry and human medicine. The aims of this study were to determine the antimicrobial susceptibility of the isolates to various antimicrobial agents and to identify some of the resistance genes. Sixty clinical isolates of each E. coli and Salmonella enterica serovar Choleraesuis collected during 1997 to 2001 from diseased pigs in Taiwan were evaluated for minimum inhibition concentration (MIC) value to antimicrobial agents. These isolates were analyzed for antibiotic resistance genes such as tem and florR by polymerase chain reaction (PCR). The correlation of virulence factor and integrons to antimicrobial resistance from the Salmonella isolates were also identified by PCR. The MIC values indicated that E. coli and Salmonella isolates had the highest activity in vitro to amikacin and ceftiofur whereas cephalothin, doxycycline, florfenicol, and gentamicin were moderately active. Flumequine was moderately active against isolates of S. enterica serovar Choleraesuis but was inactive against E. coli. Ampicillin, chloramphenicol, chlortetracycline, nalidixic acid, kanamycin, streptomycin, sulfamethoxazole, tetracycline, and trimethoprim were all inactive. The prevalence of integrons in E. coli and S. enterica serovar Choleraesuis were 97% and 95%, respectively. The prevalence of extended-spectrum β-Iactamase (tem) and florfenicol resistance (floR) in E. coli were 38% and 55%, respectively. The prevalence of tem, floR and spvC (Salmonella plasmid virulence C gene) in S. enterica serovar Choleraesuis were 60%, 25%, and 98%, respectively. The study indicated that multidrug resistance in swine E. coli and S. enterica serovar Choleraesuis isolates is predominant, and 95-97% of the isolates possess integron. A surveillance system was suggested to monitor the emergence of multidrug resistant in E. coli and S. enterica serovar Choleraesuis in swine industry.