不活化豬霍亂沙門氏菌微膠囊口服疫苗之小鼠免疫效率及保護效果評估

Immune Efficacy and Protective Effects of Oral Microencapsulated Inactive Salmonella choleraesuis Vaccine in Mice

林俊宏(Jiunn-Horng Lin) ； 翁仲男(Chung-Nan Weng) ； 蔡清恩(Chin-En Tsai) ； 陳世平(Shin-Ping Chen) ； 潘銘正(Ming-Jeng Pan)

《臺灣獸醫學雜誌》 30卷4期 (2004/12) Pp. 271-276

https://doi.org/10.7009/TVJ.200412.0271

摘要

本研究旨在利用小鼠的免疫效率與保護效果，來評估以同步噴霧乾燥法所製造的不活化豬霍亂沙門氏菌微膠囊口服疫苗。將120隻小鼠分為4組：不活化豬霍亂沙門氏菌混合油質佐劑針劑疫苗組，口服豬霍亂沙門氏菌aroA⁻減毒疫苗組，口服不活化豬霍亂沙門氏茵微膠囊疫苗組及口服PBS的對照組。在第一次免疫後，每隔10天再補強一次，其中口服aroA⁻減毒疫苗組只免疫1次，其他各組均免疫三次，在第三次免疫後7天，取10隻小鼠進行豬霍亂沙門氏菌攻毒。結果顯示口服aroA⁻減毒組保護效果最佳，雖然只有體液性IgA抗體產生，但保護率卻可達100%：微膠囊口服組雖成功誘發IgG及IgA抗體，但保護率只有60%：針劑組雖然有高濃度IgG抗體產生，但保護率最差只有20%。雖然微膠囊口服組保護效率未達百分之百，但因其所使用的為經過不活化的死菌，在使用上較為安全，具有開發的潛力。

關鍵字

豬霍亂沙門氏菌 ； 微膠囊口服疫苗 ； aroA⁻減毒口服疫苗

並列摘要

This study evaluated the immune efficacy and protective effect of an orally administered vaccine 01 Salmonella choleraesuis prepared by a co-spray drying process. 120 mice were randomly allocated into four groups. Group 1 received an SC injection of inactive S. choleraesuis vaccine with Freund's adjuvant on days 0, 10, and 20. Meanwhile, group 2 was fed with S. choleraesuis aroA⁻ oral vaccine on day 0. Furthermore, group 3 was fed with S. choleraesuis microspheres oral vaccine on days 0, 10 and 20. Finally, group four was fed a sham treatment. 10 of 30 mice in all groups were challenged with S. choleraesuis on day 27. The analytical results revealed that group 2 had the best protective rate, approaching 100%, although this group only had lower humoral immune response. Group 3 had a higher concentration of IgA than other groups but the protective rate was just 60%. Group 1 displayed a higher IgG concentration than other groups, however, the protective rate was only 20%. Although the protective rate of group 2 did not exceed 80%, this treatment had used inactive bacteria which was safe and could be considered potential for future development.

並列關鍵字

aroA⁻ oral vaccine ； microsphere oral vaccine ； Salmonella choleraesuis