Evaluation of Simple Immunodot Blotting Assay in Detecting Antibody against Newcastle Disease Virus, Egg-drop Syndrome Virus and Mycoplasma gallisepticum in Chickens

評估簡易免疫墨點試驗檢測雞新城病病毒、產蛋下降症病毒和Mycoplasma gallisepticum抗體

林茂勇(Maw-Yeong Lin) ； 鄔弘盛(Hon-Hsin Wu) ； 黃國榮(Kao-Jong Huang) ； 蔡專福(Jun-Fu Tsai) ； 蘇怡蓁(I-Chen Su)

《臺灣獸醫學雜誌》 31卷1期 (2005/03) Pp. 31-37

https://doi.org/10.7009/TVJ.200503.0031

摘要

要利用自製套組以簡化免疫墨點試驗（immundot blotting assay; IDBA）之進行，以致新套組進行之IDBA稱為簡易IDBA（SIDBA）。比較SIDBA、血球凝集抑制試驗（hemagglutination-inhibition; HI）和酵素聯結免疫吸附試驗（enzyme-linked immunosorbent assay; ELISA）檢測雞新城病病毒、產蛋下降症病毒和Mycoplasma gallisepticum（MG）抗體之敏感性和特異性。SIDBA對偵測雞新城病病毒、產蛋下降症病毒及MG抗體具高度敏感性和中等度特異性，而HI試驗則具高度特異性和中等度敏感性，ELISA則具高度敏感性和低度特異性。SIDBA如配有密度儀和電腦軟體讀取其呈色反應力價高低頗有可能取代ELISA。

關鍵字

免疫墨點試驗 ； 血球凝集抑制試驗 ； 酵素聯結免疫吸附試驗 ； 新城病病毒 ； Mycoplasma gallisepticum

並列摘要

A self-prepared kit for immunodot blotting assay (IDBA) was designed for simplifing the running of IDBA in the serological test. The test run by the noval kit is called simple IDBA (SIDBA). A comparison on the sensitivity and specificity of SIDBA in the detection of the antibody against Newcastle disease virus (NDV), egg-drop syndrome virus (EDSV) or Mycoplasma ga/lisepticum (MG) with hemagglutination-inhibition test (HI) and enzyme-linked immunosorbent assay (ELISA) in 2 trials was conducted in this experiment. The IDBA gave highly sensitive but moderately specific, the HI test gave highly specific but moderately sensitive, and the ELISA gave highly sensitive but lowly specific in detecting of NDV, EDSV or MG antibody. SIDBA could be run like ELISA with the aid of densitometer or computer program package for quantitation the density of the reaction spot.

並列關鍵字

enzyme-linked immunosorbent assay ； hemagglutination-inhibition test ； immunodot blotting assay ； Mycoplasma gallisepticum ； Newcastle disease virus