提升Leucocytozoon caulleryi及荒川庫蠓（Culicoides arakawae）培養效率

Enhancing the Culture Efficiency of Leucocytozoon caulleryi and Culicoides arakawae (Diptera: Ceratopogonidae)

余章游(Chang-You Yu) ； 張文興(Wen-Sin Chang) ； 王俊秀(Jiunn-Shiow Wang)

《臺灣獸醫學雜誌》 32卷2期 (2006/06) Pp. 129-135

https://doi.org/10.7009/TVJ.200606.0129

摘要

Leucocytozoon caulleryi, the most dominant pathogen in chicken leuoocvtozoonosis, is transmitted by Culicoides arakawae. The protozoa life is destroyed quickly at low temperature and therefore cannot be stored long term. Consequently, a highly efficient and easy approach for culturing L. caulleryi and its associated vectors is crucial to studying the pathogenesis of L. caulleryi and control strategies. The goal of this study is to enhance the efficiency of pathogen transmission, vector purification and blood feeding. C. arakawae were fed with infected blood via a chicken eggshell membrane. When infected blood cells were reconstituted with specific pathogen free sera (R) L. caulleryi infection rate and production of oocysts and sporozoites were all significantly different (p＜0.05) than those when blood cells were fed with original blood (O). Experimental chicks were inoculated with sporozoite suspensions from the above C. arakawae with a dose of 3 vectors per chick. Chick infection rates were 100% and 0% when vectors were fed to group R and group 0, respectively. Vector purity and purifying time improved significantly (p＜0.05 and p＜0.005, respectively) when a pellucid plastic bottle was applied to purify female C. arakawae. The blood-feeding success rate was enhanced significantly (p ＜ 0.05) by rubbing the breast skin of a live chicken with a cotton pellet immersed with 75% alcohol solution before the female C. arakawae fed on the chicken. In conclusion, L. caulleryi transmitted by C. arakawae was enhanced when the vectors were fed through chicken eggshell membrane with R. Blood-fed success in the vectors was helpful when chicken breast skin was rubbed with a solution of 75% alcohol and vector purification efficiency was improved by employing a pellucid plastic bottle.

關鍵字

荒川庫蠓 ； 培養效率 ； Leucocytozoon caulleryi ； 雞住血原蟲性白冠病

並列摘要

Leucocytozoon caulleryi, the most dominant pathogen in chicken leuoocvtozoonosis, is transmitted by Culicoides arakawae. The protozoa life is destroyed quickly at low temperature and therefore cannot be stored long term. Consequently, a highly efficient and easy approach for culturing L. caulleryi and its associated vectors is crucial to studying the pathogenesis of L. caulleryi and control strategies. The goal of this study is to enhance the efficiency of pathogen transmission, vector purification and blood feeding. C. arakawae were fed with infected blood via a chicken eggshell membrane. When infected blood cells were reconstituted with specific pathogen free sera (R) L. caulleryi infection rate and production of oocysts and sporozoites were all significantly different (p＜0.05) than those when blood cells were fed with original blood (O). Experimental chicks were inoculated with sporozoite suspensions from the above C. arakawae with a dose of 3 vectors per chick. Chick infection rates were 100% and 0% when vectors were fed to group R and group 0, respectively. Vector purity and purifying time improved significantly (p＜0.05 and p＜0.005, respectively) when a pellucid plastic bottle was applied to purify female C. arakawae. The blood-feeding success rate was enhanced significantly (p ＜ 0.05) by rubbing the breast skin of a live chicken with a cotton pellet immersed with 75% alcohol solution before the female C. arakawae fed on the chicken. In conclusion, L. caulleryi transmitted by C. arakawae was enhanced when the vectors were fed through chicken eggshell membrane with R. Blood-fed success in the vectors was helpful when chicken breast skin was rubbed with a solution of 75% alcohol and vector purification efficiency was improved by employing a pellucid plastic bottle.

並列關鍵字

Culicoides arakawae ； culture efficiency ； Leucocytozoon caulleryi ； leucocytozoonosis