The Use of ELISA in Classical Swine Fever Virus Antibody Monitoring in Taiwan

在臺灣地區應用酵素鍵結免疫吸附法對豬瘟抗體之監控

許岳蒼(Yueh-Tsang Sheu) ； 簡茂盛(Maw-Sheng Chien) ； 王金順(Chin-Shun Wang) ； 林正忠(Cheng-Chung Lin) ； 廖俊旺(Jiunn-Wang Liao) ； 劉正義(Cheng-I Liu) ； 李維誠(Wei-Cheng Lee)

《臺灣獸醫學雜誌》 32卷4期 (2006/12) Pp. 248-257

https://doi.org/10.7009/TVJ.200612.0248

摘要

本實驗之目的在建立臺灣地區豬瘟抗體之定量監控系統。首先，我們以免疫後豬隻血清，進行豬瘟中和抗體力價及商用阻斷型酵素鍵結免疫吸附法（blocking enzyme-linked immunosorbent assay, ELISA）檢測豬瘟抗體強度（抑制值，%）之相關性比較；結果顯示，中和抗體力價與ELISA抑制值呈現高度之線性相關（相關係數r =0.892）。為建立豬瘟疫苗免疫後之參考血清相，從14個豬瘟免疫反應良好之豬場共計300個血清樣品進行分析比較，顯示其ELISA平均抗體抑制值為104.6%。為評估移行抗體對免疫干擾之影響，取3週及6週齡不同移行ELISA抗體抑制值之豬隻以豬瘟疫苗免疫，並於免疫後6週進行血清檢測轉陽率，結果顯示血清轉陽率與移行抗體ELISA抑制值呈現二次線性迴歸相關。此外，如將血清樣品先行20倍稀釋後再進行檢測，來自兔化豬瘟疫苗免疫豬隻之ELISA檢測抑制值可以與豬瘟病毒感染恢復豬隻血清相區別。由此些結果推論，商品化之豬瘟ELISA抗體強度與血清中和抗體力價具有高度相關性，因此在台灣可應用於豬瘟抗體之定量分析與監控。

關鍵字

應用 ； 豬瘟 ； 酵素鍵結免疫吸附法 ； 監控

並列摘要

The aim of this study was to establish a quantitative classical swine fever virus (CSFV) antibody monitoring system in Taiwan. First, we used a set of serum samples from vaccinated pigs to correlate antibody titers against CSFV between a virus neutralization (VN) test and a blocking enzyme-linked immunosorbent assay (ELlSA). A good correlation (r = 0.892) was found between the VN and the ELlSA results. To establish a reference serological profile for groups with good vaccination efficacy, 300 serum samples from 14 pig farms that displayed good immune responses with lapinized Chinese C (LPC) vaccination were used in this assay. The mean ELlSA antibody inhibition value (%) was 104.6% in these pigs. To evaluate the interference of maternal antibody, three-and six-week-old pigs with different levels of maternal antibody were vaccinated with LPC vaccine, and the antibody response was assayed six weeks later. Seroconversion of the piglets displayed a quadratic regression correlation against maternal antibody levels. Serum samples of LPC-vaccinated pigs and of pigs infected with the CSFV could be differentiated by the blocking ELlSA kit if the serum samples were diluted 20-fold. In conclusion, the commercially available CSFV ELlSA kit results have a high correlation with the VN test results and can be used in the quantitative assessment of levels of CSFV antibody in Taiwan.

並列關鍵字

use ； classical swine fever ； ELISA antibody ； monitoring