- 期刊
魚類來源鏈球菌胞外產物對吳郭魚卵巢細胞株之促細胞增生效果
Mitogen Effect of Extracellular Products from Bacterial Pathogens of Fish to Tilapia Ovary (TO-2) Cell Line
摘要
The extracellular products (ECP) were proved to be the virulent factors of many bacterial pathogens. The purpose of this research was to study the cytotoxic activity of ECP(subscript s) from streptococcus and other bacteria to tilapia ovary (TO-2) cell. We compare by MU (3-[4,5-dimet-hylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) test the cytotoxic effect of ECP derived from different bacteria species including Enterococcus faecalis、Lactococcus garvieae、Streptococcus iniae、Streptococcus pyogenes、Lactococcus lactis、Aeromonas hydrophila、Edwardsiella tarda and Vibrio alginolyticus. The results appeared that the cell proliferation rate were 150% and had obvious difference when streptococcals ECP treated group compare with PBS control group, including Streptococcus spp. Lactococcus spp. and E. faecalis. From the MTT test and the results of 3H-thymidine incorporation test and cell growth curve, it appeared that the ECP from streptococcus group had the mitogenic activity and the ECP from others bacterial pathogens appeared cytotoxic effect especially the Aeromonas hydrophila.
並列摘要
The extracellular products (ECP) were proved to be the virulent factors of many bacterial pathogens. The purpose of this research was to study the cytotoxic activity of ECP(subscript s) from streptococcus and other bacteria to tilapia ovary (TO-2) cell. We compare by MU (3-[4,5-dimet-hylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) test the cytotoxic effect of ECP derived from different bacteria species including Enterococcus faecalis、Lactococcus garvieae、Streptococcus iniae、Streptococcus pyogenes、Lactococcus lactis、Aeromonas hydrophila、Edwardsiella tarda and Vibrio alginolyticus. The results appeared that the cell proliferation rate were 150% and had obvious difference when streptococcals ECP treated group compare with PBS control group, including Streptococcus spp. Lactococcus spp. and E. faecalis. From the MTT test and the results of 3H-thymidine incorporation test and cell growth curve, it appeared that the ECP from streptococcus group had the mitogenic activity and the ECP from others bacterial pathogens appeared cytotoxic effect especially the Aeromonas hydrophila.
延伸閱讀
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