- 期刊
養豬場中第二型豬環狀病毒生物性傳播途徑之探討
Biological Transmission of Porcine Circovirus Type 2 in Pig Herds
摘要
為探討豬場中非豬隻之常見生物是否可傳播第二型豬環狀病毒(porcine circovirus type2; PCV2),本試驗選定三家已感染PCV2之養豬場，捕捉場內家鼠、斑鳩、麻雀等動物，利用二氧化碳將其安樂死後解剖，取其脾臟、肝臟、肺臟、腎臟、血液、尿液及糞便等樣品進行檢測，斑鳩與麻雀則多取華氏囊樣品；犬隻則因人道考量，僅採取口鼻黏液及肛門拭子進行檢測；蚊子及蒼蠅則以每十隻為一批，直接製作組織乳劑。所有採集之樣品，均以多重引子聚合醃鏈反應(multiplex PCR; mPCR)檢測樣品中是否有PCV2的存在。共收集家鼠35例、犬隻5例、斑鳩24例、麻雀25例、蚊子26批及蒼蠅6批，經檢測結果均呈PCV2陰性反應。上述結果顯示，豬場中家鼠、犬隻、斑鳩、麻雀、蚊子及蒼蠅等動物與豬場內PCV2的傳染應無直接關聯，PCV2之傳播應是以豬隻間直接傳染為途徑。
並列摘要
Three pig herds in Pingtung County with histories of porcine circovirus type 2 infections were examined to understand whether non-pig animals act as natural reservoirs for porcine cirvovirus type 2 infections. The viral DNA was detected by multiplex polymerase chain reaction (mPCR). Thirty-five rats, 24 ringdoves, and 25 sparrows were captured and sacrificed by carbon dioxide euthanasia. Spleen, liver, lung, kidney, blood, urea, and feces samples were collected. Bursa was also collected from sparrow and ringdove. Besides, oral, nasal and rectal swabs from 5 dogs and 26 batches of mosquitoes and 6 batches of flies were collected and treated for PCV2 viral DNA detection. PCV2 DNA was not detected in all collected samples indicating that the biological transmission of PCV2 arises only among pigs, and not via other carriers such as insects and other animals living in herd.