- 期刊
錦鯉疱疹病毒傳播媒介之探討
The Research of the Transmission Vectors of Koi Herpes Virus (KHV)
摘要
本實驗室針對一般錦鯉養殖池內會出現的生物來進行同居感染試驗，以期能找出KHV在環境中的傳播途徑。同居試驗生物包括金魚(Carasslus auratus)、朱文錦(Carasslus auratus)、虎皮蛙(Rana rugulosa)、大肚魚(Gambusia affinis)、黑殼蝦(Neocaridina denticulata)及石螺(Cipangopaludina chinensis)，檢測方法採用胸腺嘧啶激酶(thymidine kinase, TK)片段基因的聚合醃鏈鎖反應(polymerase chain reaction, PCR)。結果顯示金魚、朱文錦、虎皮蛙及大肚魚皆可在與發病錦鯉共同飼養三天後檢測出陽性。進一步將這些生物與健康錦鯉共同飼養後，第三天可在錦鯉鰓部檢測出陽性反應，並使健康錦鯉魚發病死亡。由本次實驗結果可知的確有其他生物可以攜帶KHV並幫助其傳播。
並列摘要
In order to find the KHV transmission vectors in the field, we developed some cohabitation experiments to mimic the natural transmission of KHV in koi farms. The commonly-found aquatic animals in koi farms involved in the cohabitation experiments include the goldfish, common goldfish, Chinese bull frog, mosquito fish, shrimp and snail. Our results revealed that the goldfish, common goldfish, Chinese bull frog and mosquito fish showed KHV positive by the PCR method after cohabiting three days with the KHV infected koi. Those KHV-positive creatures further cohabited with the normal koi, which were examined and proved to be KHV positive three days latter. Taken together, we concluded that there are some creatures can transmit the KHV.