Transplantation of Bone Marrow Stromal Cells Improves the Regeneration of Rat Spinal Cord after Complete Transection

移植骨髓基質細胞可幫助大白鼠脊髓人工橫行切斷後脊髓的再生

曾邦彥(Pang-Yen Tseng) ； 陳春榮(Chun-Jung Chen) ； 陳建榮(Jeng-Rung Chen) ； 許慶宗(Ching-Chung Sheu) ； 林永昌(Yung-Chang Lin) ； 黃勇三(Yong-San Huang)

《臺灣獸醫學雜誌》 33卷3&4期 (2007/12) Pp. 152-161

https://doi.org/10.7009/TVJ.200712.0152

摘要

脊髓受損後若移植骨髓基質細胞可減輕脊髓受損後之功能傷害已有多人報導，但是上脊髓神經元再生的直接證據尚未被報導。本篇文章利用神經解剖追蹤及免疫化學染色的方法探討脊髓損傷後移植骨髓基質細胞是否幫助脊髓再生。神經逆向追蹤結果證實移植細胞後可幫助上脊髓神經核包括紅核、藍斑核、縫核、前庭核及網狀結構內的神經元再生。而順向追蹤的結果亦顯示移植組之大鼠大腦皮質脊髓徑之神經軸突可再生並穿過脊髓切斷區至後端。另外，結果亦證實移植入脊髓受損區之骨髓基質細胞可存活、移行、並表現出一些神經特定標定物及細胞外基質。因此，本篇文章再次證實移植骨髓基質細胞入脊髓受損區可幫助脊髓的再生。

關鍵字

骨髓質細胞 ； 再生 ； 脊髓 ； 移植

並列摘要

Several reports demonstrated that transplantation of bone marrow stromal cells could ameliorate the functional deficit after spinal cord injury. However, the evidence of the axon regeneration from supraspinal neurons has not been reported yet. Using neuroanatomical tracing technique and immunohistochemical staining methods, we studied whether transplantation of adult bone marrow stromal cells could promote axon regeneration after spinal injury. Retrograde tracing revealed that regenerated axons originated from brain stem nuclei, including red nucleus, locus coeruleus, raphe nucleus, vestibular nucleus, and reticular formation. Anterograde tracing also showed that the corticospinal axons crossed the transected sites and regenerated into caudal spinal cord stumps. Additionally transplanted marrow stromal cells survived and migrated into the spinal cord lesion sites and expressed neural markers as well as extracellular matrix. These results demonstrated that transplantation of marrow stromal cells promotes axon regeneration after spinal cord injury.

並列關鍵字

bone marrow stromal cells ； regeneration ； spinal cord ； transplantation