以高效能液相層析儀開發飼料中離子型抗球蟲藥物多重殘留分析

Development of Method for Analyzing Multiresidue of Ionophoric Coccidiostats in Feed by High Performance Liquid Chromatography

陳美雲(Mei-Yun Chen) ； 張凱鈞(Kai-Chun Chang) ； 孫豫芬(Yu-Fen Sun) ； 陳世平(Shih-Ping Chen)

《臺灣獸醫學雜誌》 37卷4期 (2011/12) Pp. 262-269

https://doi.org/10.7009/TVJ.201112.0031

摘要

本研究的目的是利用高效能液相層析儀附光二極體陣列偵測器同時分析飼料中莫孟寧素、沙利黴素、那寧素和拉薩羅四種抗球蟲藥物的檢測方法。飼料樣品萃取後經矽酸鎂固相萃取管純化後在酸性環境下以2,4-Dinitrophenylhydrazine於55℃下進行柱前衍生化反應，而後以液相層析儀梯度沖提分離，紫外光檢測波長於莫孟寧素、沙利黴素及那寧素設定為410 nm，拉薩羅為328 nm。此四種抗球蟲藥物之偵測極限值與定量極限值分別在0.08 g／mL 及0.26 g／mL 以下。添加標準品於飼料中所得到之平均回收率在62－84%之間。本研究所開發的方法經過重複性、再現性及衍生物降解測試，皆符合一般分析方法的要求。可實際應用於同時檢測四種離子型抗球蟲藥物之含量。

關鍵字

高效能液相層析儀 ； 孟寧素 ； 沙利黴素 ； 那寧素 ； 拉薩羅

並列摘要

The purpose of this study was to develop an analytical method using high performance liquid chromatography for simultaneous determination of residues of four ionophoric coccidiostats, (monensin, salinomycin, narasin, and lasalocid) in feeds. The four coccidiostats were extracted from feed and cleaned up by solid phase extraction cartridge of florisil and derivatized with 2,4-dinitrophenylhydrazine under acidic condition at 55℃. The extracted sample was detected by high performance liquid chromatography coupled with diode array detector. Wavelength for monensin, salinomycin and narasin was set as 410 nm and for lasalocid was set as 328 nm. The limits of detection and quantification were below 0.08 µg/mL and 0.26 µg/mL, respectively. The average recovery ranges of four ionophoric coccidiostats from three different spike concentrations in feed were between 61.97% and 83.68% and relative standard deviations were in the range of 2.0-7.05%. Analytical method was confirmed by repeatability, reproducibility and decay tests which were suitable for analysis of ionophoric coccidiostats in feed samples.

並列關鍵字

HPLC ； Lasalocid ； Monensin ； Narasin ； Salinomycin