大台北地區臨床就診犬隻犬瘟熱病毒感染之調查

Investigation of Canine Distemper Virus Infection in Taipei Area

蘇璧伶(Bi-Ling Su) ； 黃宣憲(Hsuan-Shian Huang) ； 劉振軒(Chen-Hsuan Liu) ； 闕玲玲(Ling-Ling Chueh)

《臺灣獸醫學雜誌》 38卷2期 (2012/06) Pp. 100-107

https://doi.org/10.7009/TVJ.201206.0100

摘要

Canine distemper virus (CDV) infection in dogs can result in subclinical infection, gastrointestinal signs, and/or respiratory signs, frequently with central nervous system (CNS) involvement, high morbidity and mortality. In order to understand more about the current CDV infection in Taipei, Taiwan, clinical specimens collected from 440 dogs clinically suspected with CDV infection were subjected to reverse transcription-nested polymerase chain reaction (RT-nPCR) analysis. The results showed that 37.7% (166/440) of the tested specimens were positive. The seasonal distribution of the infection was mainly seen in late autumn to winter (＜ 0.001). Sixty-two positive cases with multiple specimens were further investigated to compare the detection rate for different specimens. The results showed that conjunctival scraping (100.0%, 11/11) has the highest detection rate in comparison to whole blood (64.4%, 38/59) (＜ 0.05). Higher virus detection rate from specimens collected from conjunctival scrapings and ocular discharge was found in the dogs with systemic signs. This finding indicates that proper choice of specimens based on the animals clinical signs should facilitate the virus detection.

關鍵字

犬瘟熱病毒感染 ； 大台北地需 ； 反轉錄巢式聚合酶鏈鎖反應 ； 臨床症狀 ； 多重檢體 ； 檢出機率

並列摘要

Canine distemper virus (CDV) infection in dogs can result in subclinical infection, gastrointestinal signs, and/or respiratory signs, frequently with central nervous system (CNS) involvement, high morbidity and mortality. In order to understand more about the current CDV infection in Taipei, Taiwan, clinical specimens collected from 440 dogs clinically suspected with CDV infection were subjected to reverse transcription-nested polymerase chain reaction (RT-nPCR) analysis. The results showed that 37.7% (166/440) of the tested specimens were positive. The seasonal distribution of the infection was mainly seen in late autumn to winter (＜ 0.001). Sixty-two positive cases with multiple specimens were further investigated to compare the detection rate for different specimens. The results showed that conjunctival scraping (100.0%, 11/11) has the highest detection rate in comparison to whole blood (64.4%, 38/59) (＜ 0.05). Higher virus detection rate from specimens collected from conjunctival scrapings and ocular discharge was found in the dogs with systemic signs. This finding indicates that proper choice of specimens based on the animals clinical signs should facilitate the virus detection.

並列關鍵字

canine distemper virus infection ； clinical signs ； detection rate ； multiple specimeans ； RT-nested PCR ； Taipei area