A urinary catheter is the most common indwelling device in intensive care unit. Nosocomial urinary tract infections often occur in patients with with an indwelling urinary catheter. In order to reduce the incidence rate of urinary tract infection in our Medical Intensive Care Unit (MICU), we proposed an improvement project. We explored and found the most likely causes of urinary tract infection, such as lack of nursing standard of care protocol in preventing catheter-associated urinary tract infection (CAUTI), incorrect infection control practices, and lack of standardized procedures for cleaning urine specimen containers. We reviewed literature and proposed strategies for improving nursing care, including amendments to the care standard for the prevention of CAUTI and standard bath routine, providing staff continuing education , training employees to ensure proper handwashing procedures, and developing guideline for cleaning specimen containers. The post-implementation review showed that the CAUTI rate has decreased from 3.6 per mille to 0. The project has reduced infection rate, enhanced performance of infection control, and uplifted the team spirit to guide efforts to improve health care quality.