Prescriptions of Long Dan Xie Gan Tang, Yin Chen Hao Tang and WTTC Inhibit the Formation of Superoxide Radical and Scavenge Superoxide Radical in Vitro

龍膽瀉肝湯、茵陳蒿湯及WTTC體外抑制氫氧自由基形成和清除氫氧自由基之活性

黃欣欣(Hsin-Hsin Huang) ； 曾華友(Hua-Yew Cheng) ； 楊蒨旻(Chien-Min Yang) ； 林良宗(Liang-Tzung Lin) ； 林俊清(Chun-Ching Lin)

《臺灣中醫醫學雜誌》 8卷1期 (2009/03) Pp. 53-69

https://doi.org/10.30032/TJCM.200903.0006

摘要

Free radicals are reactive molecules that occur normally in organisms. However the excess generation of free radical can cause tissues and cells to be damaged, and might result in several diseases. In this study, the anti-superoxide radical formation and the superoxide radical scavenging activities of the water extract of prescriptions, Long Dan Xie Gan Tang (LDXGT), Yin Chen Hao Tang (YCHT) and WTTC, were evaluated by the xanthine/xanthine oxidase system and the cytochrome C reduction method, respectively. Results demonstrated that the water extract of the prescriptions suppressed superoxide anion formation and scavenged the radical in a dose-dependent manner. The potency on inhibiting the generation of superoxide radical was in the order of WTTC water extract (IC50=4.02±0.49 mg/ml)＞YCHT water extract (IC50=5.86±0.38 mg/ml)＞LDXGT water extract (IC50=6.52±0.38 mg/ml). The strength of superoxide radical scavenging activity was in the order of WTTC water extract (IC50=0.04±0.004 mg/ml)＞YCHT water extract (IC50=0.11±0.02 mg/ml)＞LDXGT water extract (IC50=0.55±0.18 mg/ml). In summary, the water extract of prescription LDXGT, YCHT and WTTC exhibit anti-superoxide radical activity in different magnitudes of potency in vitro.

關鍵字

龍膽瀉肝湯 ； 茵陳蒿湯 ； WTTC ； 氫氧自由基

並列摘要

Free radicals are reactive molecules that occur normally in organisms. However the excess generation of free radical can cause tissues and cells to be damaged, and might result in several diseases. In this study, the anti-superoxide radical formation and the superoxide radical scavenging activities of the water extract of prescriptions, Long Dan Xie Gan Tang (LDXGT), Yin Chen Hao Tang (YCHT) and WTTC, were evaluated by the xanthine/xanthine oxidase system and the cytochrome C reduction method, respectively. Results demonstrated that the water extract of the prescriptions suppressed superoxide anion formation and scavenged the radical in a dose-dependent manner. The potency on inhibiting the generation of superoxide radical was in the order of WTTC water extract (IC50=4.02±0.49 mg/ml)＞YCHT water extract (IC50=5.86±0.38 mg/ml)＞LDXGT water extract (IC50=6.52±0.38 mg/ml). The strength of superoxide radical scavenging activity was in the order of WTTC water extract (IC50=0.04±0.004 mg/ml)＞YCHT water extract (IC50=0.11±0.02 mg/ml)＞LDXGT water extract (IC50=0.55±0.18 mg/ml). In summary, the water extract of prescription LDXGT, YCHT and WTTC exhibit anti-superoxide radical activity in different magnitudes of potency in vitro.

並列關鍵字

Long Dan Xie Gan Tang ； Yin Chen Hao Tang ； WTTC ； superoxide radical