黑豆萃取物的抗氧化活性對健康者與心血管疾病患者LDL氧化敏感性之影響

Effect of the Antioxidant Activity of Black Soybean Extracts on LDL Oxidative Susceptibility of Health andCardiac Vascular Disease Subjects

戴文禎(Wen-Chen Tai) ； 胡雪萍(Shene-Pin Hu) ； 游添榮(Tien-Joung Yiu) ； 楊棋明(Chi-Ming Yang) ； 趙璧玉(Pi-Yu Chao)

《華岡農科學報》 31期 (2013/06) Pp. 47-69

摘要

本研究主要探討黑豆（Glycine max L. Merrilx），包括：南育3號（T3）、南育5號（T5）和市售黑豆（CBB）及市售黃豆（CSB），其水相及有機相萃取物對於健康者和心血管疾病患者（cardiac vascular disease, CVD）之低密度脂蛋白（low density liproprotein, LDL）氧化敏感性的影響與其和所含抗氧化物質之相關性。取15mL血漿分離出LDL，用體外銅離子進行氧化敏感度測試，以LDL氧化遲滯期（lag phase）與TBA可反應 （thiobarbituric acid reactive substances, TBARS）之丙二醛（malondialdehyde, MDA）產生量為指標。抗氧化物質及酵素的測定包括：金雀異黃酮（genistein）、麩胱甘肽（glutathione, GSH）、花青素（anthocyanin）、維生素E、總抗氧化狀態（total antioxidant status; TAS）、超氧歧化酶（superoxide dismutase, SOD）及麩胱甘肽過氧化酶（glutathione peroxidease, GSHPx）。結果顯示：萃取物對MDA 生成的抑制性，包括對健康者與心血管疾病患者，在240分鐘時，水相萃取物以T3抑制性最佳，而有機相萃取物以T3及T5的抑制性最佳。黑豆與黃豆水相萃取物MDA抑制百分比與所含GSH、genistein、anthocyanin和TAS成正相關；而有機相萃取物MDA抑制百分比與所含維生素E和genistein成正相關。同時水相與有機相萃取物，皆能延長心血管疾病患者與健康者其LDL氧化遲滯期約為對照組LDL氧化遲滯期的2-2.67倍。以T3水相萃取物對心血管疾病患者LDL氧化遲滯期的延長效果最佳；而T3有機相萃取物對健康者LDL氧化遲滯期的延長效果最佳，亦即對心血管疾病患者與健康者LDL氧化遲滯期的最佳延長萃取相不同。

關鍵字

黑豆 ； 遲滯期 ； 丙二醛 ； 金雀異黃酮 ； 花青素

並列摘要

The objective of studies is to determine the antioxidant activity of Glycine max L. Merrilxfrom water and organic solvent extracts of Tainan #3 (T3), Tainan #5 (T5), commercial black soybean (CBB),and commercial soybean (CSB) in LDL (low density lipoprotein) oxidative susceptibility by LDLoxidized lag phase and thiobarbituric acid reactive substances for malondialdehyde (MDA) productionassay in health and cardiac vascular disease (CVD)subjects. Second, to assay the activities of antioxidative enzymes and concentrations of antioxidant of T3, T5, CBB, and CSB in related toLDLoxidative susceptibility. LDL was isolated from fifteen millilitersof plasma and treated with Cu^(2+) for oxidative susceptibility assay included LDL lag phase and MDA measurement in vitro. The antioxidants and enzymes including glutathione (GSH), anthocyanin, genistein, vitamin E, total antioxidant status (TAS), superoxide dismutase(SOD), and glutathione peroxidase (GSHPx) were measured. The results shown that water and organic extracts of black soybeans and soybean significantly inhibited MDA formation in health and CVD subjects. The % inhibition of T3 was significantly higher than T5, CBB, and CSB in water extracts, while the % inhibition of T3 and T5 were significantly higher than CBB and CSB in organic extracts. In addition, water extract of four treatments in inhibiting of MDA formation significantly positive correlated withthe contents of GSH, genistein, anthocyanin, and TAS. However, organic extract of four treatments in inhibiting of MDA formation significantly positive correlated withthe contents of vitamin E and genistein. Hence, water and organic extract of four treatments also could prolong the lag phase in bothCVD and health treated groups by 2-2.67 fold as compared to the lag phase of control group. Particularly, T3 water extract was most effective on prolonging of the lag phase in CVD treated group and T3 organic extract was most effective on prolonging of the lag phase in health treated group. In conclusion, water or organic extracts had different efficacy on prolonging the lag phase of LDL oxidation in CVD and health groups.

並列關鍵字

black soybean ； lag phase ； malondialdehyde ； genistein ； anthocyanin