沉香樹（Aquilaria crassna）療傷組織誘導與繼代培養之探討

Callus induction and subculture analysis in Aquilaria crassna

蕭履慶(Lu-Ching Hsiao) ； 陳淑玲(Shu-Ling Chen) ； 林冠宏(Kung-Huan Lin) ； 江志明(Chih-Ming Chiang)

《華岡農科學報》 41期 (2018/06) Pp. 33-42

摘要

沉香(Agarwood)為瑞香科常綠喬木植物沉香樹中所蘊育而成含有黑色樹脂的木材，主要作為珍貴中藥使用之外，沉香的濃郁芳香使其成為世界上名貴的香料之一。本實驗研究目的在探討沉香樹癒傷組織培養之適合誘導及培養條件，作為日後誘導生產「沉香」成分的基礎研究。以MS為基本培養基，在不同植物生長激素6-benzylaminopurine (BA, 苯甲酸嘌呤)與naphthaleneacetic acid (NAA, 萘乙酸)下，觀察對沉香樹樹癒傷組織誘導的情形，建立其最適合癒合組織誘導及培養條件。首先，將沉香種子胚進行無菌播種，生成無菌苗做為實驗材料，再以不同濃度BA及NAA組合的培養基作為誘導癒傷組織的試驗培養基。結果顯示BA 4.0 μM、NAA 4.0μM的效果最佳。而實驗中發現，當固定NAA濃度時，隨著BA濃度遞增時，癒傷組織誘導率或細胞增殖率有遞增的趨勢；而以BA濃度2.0μM以上較為適當，太低濃度的BA會使癒傷組織誘導率減低。因此，本研究首次試驗出繼代培養沉香樹癒傷組織之條件，，未來將進一步探討利用沉香樹組織細胞能否用於生產「沉香」成分之可行性研究。

關鍵字

沉香 ； 6-苯甲酸嘌呤 ； 癒傷組織的誘導與繼代 ； 萘乙酸

並列摘要

Agarwood is an expensive resinous product extracted from Aquilaria species of the family Thymalaeaceae. It has been used in the production of high quality of perfumes as well as in traditional medicines. The objective of this experiment was to investigate the suitable induction and culture conditions for the callus culture of A.crassna as a basic research for the induction of production of Agarwood components in the future. The MS medium was used as a basic medium supplied with different concentrations of plant growth hormones 6-benzylaminopurine (BA) and naphthaleneacetic acid (NAA) for the callus induction of A. sinensis, and the conditions for induction and culture of the most suitable callus were established. The seed embryos of A. sinensis were aseptically sowed to generate sterile seedlings, and were used as experimental materials. The culture medium with different concentrations of BA and NAA were used as the test medium for inducing callus. The results show that BA 4.0 μM and NAA 4.0 μM had the best effect on callus induction. When the concentration of NAA was fixed, the callus induction rate or cell proliferation rate was increased as the BA concentration increased above 2μM. The callus induction rate was reduced as the concentration of BA was lower than 2μM. In conclusion, the conditions for the effective induction and subculture of agarwood callus were tested, and the feasibility of using agarwood tissue cells for the production of agarwood components will be explored in the near future.

並列關鍵字

Agarwood ； 6-benzylaminopurine ； Callus induction and subculture ； Naphthaleneacetic acid