以試管培養法探討添加嗜甲烷菌、乳酸菌、亞麻仁油與亞麻仁籽粉對鵝盲腸內容物甲烷產生量之影響

The Effects of in Vitro Addition of Methanotropic Bacteria, Lactic Acid Bacteria, Linseed Oil and Flaxseed Meal on Methane Emission in Cecal Contents of Geese

陳盈豪(Yieng-How Chen) ； 羅能文(Neng-Wen Lo) ； 王淑音(Shu-Yin Wang)

《華岡農科學報》 44期 (2019/12) Pp. 113-124

摘要

本試驗之目的係探討添加嗜甲烷菌、乳酸菌、亞麻仁油與亞麻仁籽粉對鵝盲腸內容物甲烷排放之影響。以鵝為試驗動物進行三個試驗，分別添加嗜甲烷菌、乳酸菌、亞麻仁油與亞麻仁籽粉於鵝盲腸內容物中，以試管培養法探討其對甲烷排放量之影響。結果顯示，試驗一：以100μL之1.25 × 10^6 cells/mL嗜甲烷菌在鵝盲腸內容物以試管培養經24hr後，並無顯著降低鵝盲腸內容物之甲烷排放量，然而添加同劑量之乳酸菌於鵝盲腸內容物卻顯著的增加甲烷排放量（P＜0.05）。試驗二：鵝盲腸內容物添加亞麻仁油之甲烷排放量隨亞麻仁油量之增加而減少；當盲腸內容物添加亞麻仁油量達0.1g時，即有顯著的降低其甲烷排放量（P＜0.05），此數值與對照組相比較，則下降21.3%。試驗三：添加0.5、1、1.5與2g亞麻仁籽粉使鵝盲腸內容物甲烷排放量增加，但並未呈劑量反應，於添加1g時達最高排放量，之後隨添加量增高而排放量降低。綜合上述，試驗一低濃度乳酸菌增加鵝盲腸內容物甲烷排放量，然而低濃度嗜甲烷菌對鵝盲腸內容物甲烷排放量則無影響，試驗二亞麻仁油有降低鵝盲腸內容物甲烷生成量之效果，試驗三添加亞麻仁籽粉無降低鵝盲腸內容物甲烷排放量。本研究揭示於鵝之飼料中添加亞麻仁油可能降低鵝隻之甲烷排放量而對減緩溫室效應有相當之成效。

關鍵字

嗜甲烷菌 ； 亞麻仁油 ； 亞麻仁籽 ； 鵝 ； 盲腸內容物 ； 甲烷

並列摘要

The purpose of this study was to investigate the effects of addition of methanotropic bacteria, lactic acid bacteria, linseed oil and flaxseed meal on the methane emission in cecal contents of geese. Cecal contents of geese allotted to three trials were used to study the effects of in vitro supplemented methanotropic bacteria, lactic acid bacteria, linseed oil and flaxseed meal to the contents of cecal cecum on methane emissions. The results showed that adding 100 μL of 1.25 × 10^6 cells/mL methanotropic bacteria in the cecal contents of the geese in the test tube during 24 hrs incubation did not significantly reduce the methane emissions; however, adding the same dose of lactic acid bacteria in cecal contents of goose significantly increased methane emissions (P ＜ 0.05; Trial 1). In Trial 2, the methane emissions from the cecal contents added with the linseed oils decreased with the raised amount of linseed oil added. When the cecal contents were added with 0.1 g of linseed oil, a significant reduction (21.3 %) in methane emissions was observed (P ＜ 0.05) as compared to the control group. In Trial 3, adding 0.5, 1, 1.5, and 2 g of flaxseed meal increased the methane emissions from the contents of the cecum of the geese, but it did not show a dose response. The maximum emission was reached when 1 g of flaxseed meal was added, and then the emissions decreased with the increase of the amount added. Taken together, addition of a low concentration of lactic acid bacteria into the cecal contents increased the methane emissions; with the same dose and concentration of methanotropic bacteria added no effect on the methane emissions was observed. The linseed oil had the effect of reducing the methane production of the goose cecal contents; however, adding flaxseed meal did not reduce methane emissions from cecum contents of geese. It is suggested that the addition of linseed oil to the goose feed may reduce the methane emission and contribute to the reduction of greenhouse gas emission.

並列關鍵字

cecal contents ； flaxseed meal ； geese ； linseed oil ； methanotropic bacteria ； methane emission