Effects of Human Spleen's RNA on Peritoneal Macrophage in Irradiated Mice

本文旨在於評估異源性人類核糖核酸經皮下注射移植於輻射小鼠對於骨髓細胞及腹膜巨噬細胞之輻射保護效應，人類核糖核酸劑量為每週二次，每次劑量為0.16 mg / kg，總劑量共處理十次，射源劑量率為107.8 cGy/min，給予單一全身輻射劑量為500 cGy，骨髓細胞明顯受到輻射抑制，人類核糖核酸能明顯輔助骨髓細胞之恢復與免疫競爭力，骨髓細胞經輻射後之回復與給藥時程有其相關性，巨噬細胞之吞噬活性比與人類核糖核酸總劑量更有依賴性存在，骨髓細胞於輻射後恢復潛力在二大實驗組沒有差異性，但在輻射後造血組織的回復上確有顯著性加強作用，巨噬細胞之吞噬指數與骨髓細胞回復的現象，在不同組別上展示出不同的趨勢，吞噬指數與控制組有5~9 倍之明顯差異，研究結果顯示有一些免疫中間媒介物質可能受核糖核酸的誘發而產生，進而誘發增強巨噬細胞吞噬指數的能力。

關鍵字

人類核糖核酸；腹膜巨噬細胞；吞噬指數

並列摘要

The radioprotective effectiveness of bone marrow cells and peritoneal macrophage on transplantation immunity of human spleen’s RNA (iRNA) from xenogenic sources by subcutaneous injection in irradiated mice was investigated. The dosage of human spleen’s RNA was 0.16 mg/kg/day twice a week, and the total dose was treated with human spleen’s RNA 10 times. Whole body irradiation with a single dose of 500 cGy at a dose rate of 107.8 cGy/min was used. Bone marrow cells were evidently inhibited after radiation treatment, and iRNA could help the recovery and increase the immune competence on bone marrow cells. The recovery of bone marrow cells after irradiation had relations with given scheme of drug, and the relative ratios of phagocytosis were dependent on the total dose of human spleen’s RNA. The potential of recovery on phagocytosis in all groups was not different, but there is significant enhancement on the recovery of hematopoietic tissues after irradiation. The recovery tendency of bone marrow cells and phagocytosis indexes treated by iRNA and irradiation increased quickly, and the indexes was about 5 to 9 times more than control group apparently. Some immediators may be induced by human spleen’s RNA to enhance and increase the phagocytosis index after human spleen’s RNA and irradiated treatments.

並列關鍵字

Peritoneal macrophage ； Human spleen's RNA ； Phagocytosis index

國際替代計量

Effects of Human Spleen's RNA on Peritoneal Macrophage in Irradiated Mice

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