神祕果萃取物對人類癌症（皮膚癌）抑制之研究

Anticancer (Human Melanoma) Proliferation Activities of Synsepalum Dulcificum Extracts

陳中一(Chung-Yi Chen) ； 郭柏麟(Po-Lin Kuo) ； 楊偉伶(Woei-Ling Yang) ； 王昭仁(Zhao-Ren Wang) ； 王惠民(Hai-Min Wang)

《美容科技學刊》 6卷1期 (2009/03) Pp. 23-32

https://doi.org/10.30001/JIES.200903.0005

摘要

本研究目的為探討神祕果對人類癌症細胞之抑制成效，特別是對抑制皮膚癌之效果。
先將神祕果果肉經100%甲醇與氣仿萃取，製得萃取物，再進行多項人類癌細胞抑制效能評估。本實驗為第一次神祕果萃取物之生物活性研究。神祕果萃取物對於人類皮膚、肺臟、肝臟、前列線、子宮頸都有明顯抗癌細胞增生之效用。此外，亦對骨頭、膀胱和乳腺之癌細胞株分別評估，發現同樣能抑制其增生。有趣的是，藉由MTT細胞存活測試，神祕果萃取物在兩株黑色素瘤細胞株的擴散上均有特別明顯的抑制作用。分別檢測其對人類癌症細胞抑制成效，發現神祕果的甲醇萃取物表現出強抑制效力。在對皮膚癌細胞(A375.S2/MeWo)的研究中，其IC50為72.7/49μg/ml。
從本實驗給果，我們相信神秘果莘取物為具有潛能之醫學美家藥劑，能有效抑制人類皮膚癌的生食神秘果萃取物使用於化粧品原料或相關產品上將能增加其應用價值。

關鍵字

神秘果 ； 人類黑色素瘤細胞 ； 抑制

並列摘要

The purpose of this study is to survey the inhibition activities of Synsepolun dulcificum on human cancer cells, especially on skin cancer.
The fruit of S. dulcificum was extracted with methanol (MeOH) and CHCl3 respectively, and evaluate their effects on inhibiting human cancer cells. This was the first time to analyze the bioactivities of this plant species. The data of antiproliferative effects of S. dulcificum extracts on human skin, liver, lung, prostate, cervical, bone bladder, and breast cancer cell lines were also investigated. Interestingly, through MTT cell viability assay, S. dulcificum extracts showed strong inhibition activities only on the proliferation of melanoma cell lines. We detected their inhibition effects on various human cancer cells and found extracts of S. dulcificurn showed strong inhibition activities. Its IC50 is 72.7/49.4 t g/ml on skin cancer cells (A375.S2/MeWo).
From these experiments, we believed that extracts from S. dulcificum were potentially potent new medical cosmetology agents, with anti-skin cancer activities.

並列關鍵字

Synsepalum dulcificum ； human melanoma cell ； inhibition

參考文獻

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