植物乳桿菌發酵液作為皮膚保養品之安全性與功效性評估

Safety and Efficacy Evaluation of Lactobacillus plantarum GKM3 Fermented Filtrate as Skin-care Product

陳婉屏(Chen, Wan-Ping) ； 蔡侑珊(Tsai, You-Shan) ； 陳彥博(Chen, Yen-Po) ； 顏峰霖(Yen, Feng-Lin) ； 陳勁初(Chen, Chin-Chu)

《美容科技學刊》 19卷2期 (2022/12) Pp. 25-40

https://doi.org/10.30001/JIES.202212_19(2).0002

摘要

從傳統醃漬物分離出之植物乳桿菌Lactobacillus plantarum GKM3經培養後，其發酵液依「化粧品衛生管理條例」以體外試驗進行安全性（細胞毒性、皮膚刺激性）及功效性評估。安全性試驗結果顯示，GKM3發酵液對皮膚細胞不會引起發炎或毒性反應，是安全的皮膚外用成分。功效性試驗結果顯示，GKM3發酵液可提高細胞能量ATP含量及抗老基因Cisd2表現量，推測GKM3發酵液可有效清除自由基，維持細胞能量代謝，延長細胞壽命。臨床試驗上，肌膚敏感組與肌膚衰老組使用含有GKM3發酵液之保養品12週後，皆顯示臉部肌膚彈性顯著提升，且肌膚衰老組之皺紋獲明顯改善。綜合上述結果，GKM3發酵液是安全且可作為抗老功效之皮膚保養品成分。

關鍵字

後生元 ； 抗皺 ； 皮膚彈性 ； 面部護理

並列摘要

Lactobacillus plantarum GKM3 isolated from traditional pickles was cultured, and its fermented filtrate was evaluated in vitro in accordance with the ＂Statute for Control of Cosmetic Hygiene＂ for safety (cytotoxicity, skin irritation) and efficacy assessments. The results of the safety test showed that GKM3 fermented filtrate did not cause inflammation or cytotoxicity to the skin cells, and is a safe topical ingredient for skin. The efficacy test results showed that GKM3 fermentation filtrate could increase the cellular energy ATP content and the expression of anti-aging gene Cisd2. It is speculated that GKM3 fermented filtrate may effectively scavenge free radicals, maintain cellular energy metabolism, and prolong cell lifespan. In clinical trials, a significant improvement on the skin elasticity was observed in both the sensitive skin group and the skin aging group after using the skin care product containing GKM3 fermented filtrated for 12 weeks. Besides, the reduction of the wrinkles in skin aging group was significantly observed. Based on the above results, GKM3 fermented filtrate is safe and can be used as a skin care ingredient for anti-aging effect.

並列關鍵字

postbiotics ； anti-wrinkle ； skin elasticity ； facial nursing

參考文獻

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