The purpose of this study was to explore the effect of the different training samples, learning rate and numbers of hidden layers on the classified accuracy when using Learning vector Quantization to analysis. When the number of the training samples were on the two numbers, 200 and 250, the classified accuracy was irregular, and the difference between these two number was not significant. As a result, the classified accuracy of training samples were increasing while the training samples were increasing. By using the simulation testing method to compare the accuracy of learning rate, in any kind of mixed samples, when the learning rate was on 0.1, the classified accuracy was the worst; however the classified accuracy from other learning rates was irregular. When the number of hidden layers was 2, the classified accuracy was the best in any kind of mixed samples.