- 期刊
Behaviour of proteins on reversed-phase supports during highperformance liquid chromatography and prediction of protein retention times on different Zorbax stationary phases
Valkude käitumine kõrgefektiivses vedelikkromatograafias pöördfaaskandjatel ja retentsiooniaegade ennustamine erinevate Zorbaxi tüüpi statsionaarsete faaside korral
並列摘要
The separation of high-molecular compounds under isocratic conditions is very difficult, if possible at all, and thus gradient elution is needed. The theory of gradient elution for small molecules is well established; however, its applications to reversed-phase gradient separations of biopolymers are not straightforward because of specific problems, such as slow diffusion, limited accessibility of the stationary phase for larger molecules, or possible sample conformation changes during the elution. The first step of our study was the determination of the experimental data, and then these data were used to predict gradient retention times. High performance liquid chromatography was used to investigate the reversed-phase chromatographic behaviour of four proteins. The influence of experimental parameters was examined using a water/organic solvent/trifluoroacetic acid system. Chromatographic results from four Zorbax stationary phases supports were comparable.
延伸閱讀
- Kannike, A. (2006). KULTUURILISE JÄRJEPIDEVUSE HOIDMINE KODUSFÄÄRIS. NÕUKOGUDE EESTI NÄIDE. Acta Historica Tallinnensia, 10(1), 212-229. https://www.airitilibrary.com/Article/Detail?DocID=17367476-200612-201011020001-201011020001-212-229
- Rotkaja, O., Golushko, J., & Mekss, P. (2015). Proteiinide käitumine pööratud faasi kolonnis kõrgrõhu vedelikkromatograafia ajal C18 statsionaarses faasis. Proceedings of the Estonian Academy of Sciences, 64(1), 36-42. https://doi.org/10.3176/proc.2015.1.05
- Antonov, M., Hussainova, I., Pirso, J., Juhani, K., & Viljus, M. (2010). Oksüdeerimise mõju titaankarbiidkermiste kulumisintensiivsusele liivakeskkonnas. Estonian Journal of Engineering, 16(4), 264-272. https://www.airitilibrary.com/Article/Detail?DocID=17367522-201012-201012070005-201012070005-264-272
- Widmaier, T., Uusimäki, J., Kuosmanen, P., Juhanko, J., & Väänänen, P. (2007). Tugivaltside mitteümarlihvimine valtsimisjõu kõikumise vähendamiseks. Proceedings of the Estonian Academy of Sciences.Engineering, 13(2), 168-179. https://www.airitilibrary.com/Article/Detail?DocID=14060175-200762-201011040081-201011040081-168-179
- Klevtsov, I., Dedov, A., & Molodtsov, A. (2009). Aurukatlateraste tõmbetugevuse ja voolepiiri mõõtmine kuuliga surumise ning tõmbekatse meetodil. Estonian Journal of Engineering, 15(2), 99-107. https://www.airitilibrary.com/Article/Detail?DocID=17367522-200906-201011040089-201011040089-99-107