- 期刊
The Expression of MAGE Genes in Human Breast Carcinoma in Taiwan
黑色素瘤抗原(MAGE)基因在浸潤腺乳癌腫瘤組織上之表現
摘要
Background and purpose: Some human neoplasms express antigens that can be used as targets for tumor-specific immunotherapy. Cancer testis antigens (CTAs) such as melanoma antigen (MAGE) are expressed in many tumors but are silent in normal tissues except for testis and placental tissue. Such a specific distribution pattern makes them promising targets for possible cancer immunotherapy. Methods: Using 2 human breast cancer cell lines for positive control, tumor samples collected from 23 patients who had undergone surgical treatment for breast infiltrative ductal carcinoma at Cathay General Hospital, Taipei, were examined. After isolation of total RNA, reverse-transcriptase polymerase chain reaction (RT-PCR) was carried out to determine the presence of mRNAs of 4 genes in the MAGE family which have recently been identified: MAGE-1, MAGE-2, MAGE-3, andMAGE-12. The PCR products were further separated on agarose gels and visualized by ethidium bromide staining. Results: Twenty-one of 23 samples were positive for MAGE mRNA. The positive expression rates of MAGE-2 (52.2%), MAGE-3 (34.8%), and MAGE-12(56.5%) were significantly (p＜0.01) higher than the expression rate of the MAGE-1 (0%) gene. Conclusion: Our data suggest that MAGE-2, MAGE-3, and MAGE-12, but not MAGE-1, are possible candidates for immunotherapy for Taiwanese breast cancer patients. In addition, multivalent cancer vaccines are required for recognizing human breast cancer cells.
並列摘要
Background and purpose: Some human neoplasms express antigens that can be used as targets for tumor-specific immunotherapy. Cancer testis antigens (CTAs) such as melanoma antigen (MAGE) are expressed in many tumors but are silent in normal tissues except for testis and placental tissue. Such a specific distribution pattern makes them promising targets for possible cancer immunotherapy. Methods: Using 2 human breast cancer cell lines for positive control, tumor samples collected from 23 patients who had undergone surgical treatment for breast infiltrative ductal carcinoma at Cathay General Hospital, Taipei, were examined. After isolation of total RNA, reverse-transcriptase polymerase chain reaction (RT-PCR) was carried out to determine the presence of mRNAs of 4 genes in the MAGE family which have recently been identified: MAGE-1, MAGE-2, MAGE-3, andMAGE-12. The PCR products were further separated on agarose gels and visualized by ethidium bromide staining. Results: Twenty-one of 23 samples were positive for MAGE mRNA. The positive expression rates of MAGE-2 (52.2%), MAGE-3 (34.8%), and MAGE-12(56.5%) were significantly (p＜0.01) higher than the expression rate of the MAGE-1 (0%) gene. Conclusion: Our data suggest that MAGE-2, MAGE-3, and MAGE-12, but not MAGE-1, are possible candidates for immunotherapy for Taiwanese breast cancer patients. In addition, multivalent cancer vaccines are required for recognizing human breast cancer cells.
延伸閱讀
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