一氧化氮在大鼠皮膚血管顯影劑滲漏時所扮演的角色

Role of Nitric Oxide Production in Increased Vascular Leakage in Rat Skin Induced by Contrast Media

陳偉琪(Wei-Chi Chen) ； 曾文昌(Wen-Chang Tszen) ； 賴世育(Shieh-Yu Lai) ； 王永成(Yung-Cheng Wang)

《輔仁醫學期刊》 8卷4期 (2010/12) Pp. 209-222

摘要

依照流行病學研究指出，自由基以及一氧化氮在外傷性所造成血管破裂或滲漏等疾病的因果關係中，扮演了一個重要的角色。本實驗經由大鼠靜脈注射染劑Evans blue(EB)，接著於鼠背皮膚皮內局部注射生理食鹽水、離子或是非離子顯影劑，並給予不同的醫療處理方式之後，粹取組織中的EB以測量血管滲透度。實驗結果顯示，顯影劑注射時間越長，血管滲漏的程度越高。當給予冰敷的醫療處置之下，可以將顯影劑所造成血管滲漏濃度過高的情況壓抑下來；當給予熱敷的醫療處置之下，則反而助長顯影劑所造成血管滲漏濃度升高。而saline注射（控制組），僅造成非常少量的血管滲漏。另外，依照膠體碳滲漏標記以及組織形態學實驗方面，血管滲漏的實驗結果顯示：顯影劑注射的時間越長，膠體碳所呈現出的血管滲漏情形越高，且血管被破壞後；紅血球外滲的情形越嚴重。當給予冰敷的醫療處置之下，可以將顯影劑所造成血管滲漏引發紅血球滲漏過高的情況壓抑下來；當給予熱敷的醫療處置之下，則反而助長紅血球滲漏濃度升高。至於在免疫組織化學染色方面，以nitrotyrosine的存在當作指標。實驗結果顯示：顯影劑滲漏時間越長，nitrotyrosine的呈色就越深；當給予熱敷的醫療處置之下，同樣可以觀察到nitrotyrosine的呈色越深，當給予冰敷的醫療處置之下，亦無法抑制nitrotyrosine的呈色。綜合以上結果顯示：顯影劑滲漏於皮膚皮內的時間越長，NO的產生就越多，血管被破壞滲漏的情形就越嚴重。本研究的結論是：NO的過量產生參與顯影劑所造成的血管傷害及滲漏。

關鍵字

一氧化氮 ； 血管破裂或滲漏

並列摘要

A correlation between radiation and vascular disease has been established through epidemiological studies. Previous studies have shown that the increased vascular injury and inflammation is induced by radiation exposure. However, the underlying mechanism remains unclear. Previous studies indicate that increased vascular injury by stimuli including endotoxin induced acute inflammation can be diminished by treatment with a nitric oxide syhthase (NOS) inhibitor, indicating that nitric oxide (NO) is required for stimuli to cause vascular leakage. Therefore, the goal of the present studies is to investigate whether NO plays a role in increased vascular injury induced by radiation. Rats were intravenously injected with radiation contrast media.The results (rats, n=6/group ) of contrast media leakage assay showed that 0.1 c.c. of contrast media intravenously injected to rat, attenuated increased vascular injury and inflammation induced by C.M leakage. Five hours after C.M intravenously injected, the vascular leakage induced by C.M leakage. During the intervals of 24 hours, C.M intravenously injected, the vascular leakage induced by C.M leakage. The H&E staining technique also demonstrated a time-dependent increases of vascular labeling in the tissues examined. In addition, we also investigated the NO production after contrast media injected in rats with nitrotyrosine staining. The IHC staining technique results showed that the NO production was significantly increased after contrast media injection and reached a maximum at 24 hours after C.M leakage. The increased NO production in rat skin was correlated with the increased leakage and inflammation of blood vessels. In conclusion, the results indicate that NO plays important a roles in tissue damage (vascular permeability) caused by contrast media leakage in rat skin in vivo.

並列關鍵字

nitric oxide ； vascular leakage