Impact of Active Compound Salidroside from Rhodiola Rosea on Bladder Cancer

探討紅景天活性化合物紅景天苷對膀胱癌的保護作用

吳俊賢(Chun-Hsien Wu) ； 陳忠賢(Chung-Hsien Chen) ； 謝佩坊(Pei-Fang Hsieh) ； 吳振宇(Richard Chen-Yu Wu) ； 陳毓騏(Yu-Chi Chen) ； 洪志勳(Chih-Shin Hung) ； 楊堉麟(Yu-Lin Yang) ； 林嘉祥(Victor C. Lin)

《輔仁醫學期刊》 17卷1期 (2019/03) Pp. 9-21

https://doi.org/10.3966/181020932019031701002

摘要

Background and purpose: Salidroside, extracted from Rhodiola rosea, has been reported to possess anti-tumor effect. However, the therapeutic effects of Salidroside on bladder cancer have not been investigated. This study focused on the effects and possible mechanisms of Salidroside on human bladder cancer in vitro. Methods: The human bladder cancer cell line, T24, was incubated with various concentrations of Salidroside (12.5, 25 and 50 ug/ mL) for 24 hours, followed by serial biological examinations. Cell viability, proliferation and toxicity were accessed by MTT, trypan blue exclusion assay and LDH assay. Cell migration was accessed by wound healing assay. ELISA, western blot and immunofluorescence staining were used to detect the expression of TGF β1/smad signaling related proteins and epithelial- mesenchymal transition (EMT). Results: Salidroside treatment of T24 cells significantly inhibited cell proliferation and cell migration. In addition, Salidroside significantly reduced the expression of TGF-β1 and downstream signaling in T24 cells. TGF-β1 downstream signal regulators Smad 2/3 was reduced, while the inhibitor Smad 7 was increased. Further investigating the EMT markers was performd, which showed that Salidroside decreased EMT through decreasing the expression of α-SMA and increasing expression of E-cadherin. Conclusion: In the present study, Salidroside was found to show anticancer effects on bladder cancer T24 cells. These effects were demonstrated by suppressing cell proliferation, tumor invasion and EMT. The underlying mechanisms may be associated with modulating EMT process by down-regulating TGF β1/Smad pathway.

關鍵字

bladder cancer ； Salidroside ； Rhodiola rosea ； TGF-β1 signaling ； Epithelial-mesenchymal transition

並列摘要

背景和目的：據文獻報告，紅景天活性化合物，紅景天苷，具有抗腫瘤作用。然而，紅景天苷在膀胱癌中的治療效果尚不清楚。因此，本研究的重點是探討紅景天苷對膀胱癌細胞的抑制作用及可能機制。方法：將膀胱癌細胞株T24與不同濃度的紅景天苷進行一系列生物學檢查，了解紅景天苷對膀胱癌細胞株T24的抑制生長作用及其對癌細胞上皮間質轉化（EMT）的影響及其下游調節蛋白表現。結果：發現紅景天苷顯著抑制細胞增殖和細胞遷移。此外，紅景天苷顯著降低T24膀胱癌細胞株上皮間質轉化，使上皮性質表現增強。結論：在本研究中，紅景天苷被發現對膀胱癌T24細胞株具有抗癌作用。通過TGFβ1/Smad途徑，調節癌細胞上皮間質轉化過程，抑制細胞增殖，腫瘤侵襲和增強上皮性質表現。

並列關鍵字

紅景天苷 ； 膀胱癌 ； 上皮間質轉化（EMT）