重緣葉馬尾藻萃取物之抗氧化特性及其對人類大腸癌細胞株HT29細胞之增生抑制作用

Antioxidant Properties and Antiproliferative Activity in Human Colorectal Adencarcinoma (HT29) Cells of the Brown Alga Extracts, Sargassum duplicatum (J. Agardh)

李民賢(Min-Hsien Lee) ； 蔡伊婷(Yi-Ting Tsai) ； 羅俊光(Jiunn-Guang Lo)

《健康管理學刊》 10卷1期 (2012/06) Pp. 34-45

https://doi.org/10.29805/JHM.201206.0004

摘要

The antioxidant properties and antiproliferative activity of the brown alga, ”Sargassum duplicatum” C. Agardh, were tested in this study. S. ”duplicatum” were extracted with hot water (WE), ethanol (EE), and supercritical carbon-dioxide fluid (SFE), respectively. The total phenolic contents, flavonoid contents, and antioxidant activities, including DPPH (α, α-diphenyl-2-pricrylhydrazyl) radical scavenging and reducing power were assayzed. And, the anti-proliferative activity of S. ”duplicatum”, extracted from hot water, ethanol, and supercritical carbon-dioxide fluid, were tests in human colorectal adencarcinoma cell line HT29 using the MTT assay. The result showed that the WE and SFE of S. ”duplicatum” have higher phenolic compounds and strong antioxidant activities than EE (p＜0.05); The proliferation of HT29 cells was significantly inhibited at 48hr after exposure to the SFE, EE and WE, and the antiproliferative effect was highest in cells treated with SFE, follow by EE, and the lowest in cells treated WE, the differences among all groups were significant (p＜0.05). This study suggested that brown alga, S. ”duplicatum” is a useful healthy seafood having antioxidant properties and antiproliferative activity.

關鍵字

重緣葉馬尾藻 ； 超臨界二氧化碳流體 ； 抗氧化特性 ； 人類大腸癌細胞株HT29 ； 細胞增生抑制活性

並列摘要

The antioxidant properties and antiproliferative activity of the brown alga, ”Sargassum duplicatum” C. Agardh, were tested in this study. S. ”duplicatum” were extracted with hot water (WE), ethanol (EE), and supercritical carbon-dioxide fluid (SFE), respectively. The total phenolic contents, flavonoid contents, and antioxidant activities, including DPPH (α, α-diphenyl-2-pricrylhydrazyl) radical scavenging and reducing power were assayzed. And, the anti-proliferative activity of S. ”duplicatum”, extracted from hot water, ethanol, and supercritical carbon-dioxide fluid, were tests in human colorectal adencarcinoma cell line HT29 using the MTT assay. The result showed that the WE and SFE of S. ”duplicatum” have higher phenolic compounds and strong antioxidant activities than EE (p＜0.05); The proliferation of HT29 cells was significantly inhibited at 48hr after exposure to the SFE, EE and WE, and the antiproliferative effect was highest in cells treated with SFE, follow by EE, and the lowest in cells treated WE, the differences among all groups were significant (p＜0.05). This study suggested that brown alga, S. ”duplicatum” is a useful healthy seafood having antioxidant properties and antiproliferative activity.

並列關鍵字

Sargassum duplicatum ； supercritical carbon-dioxide fluid ； Antioxidant properties ； human colorectal adencarcinoma cell line HT29 ； Antiproliferative activity