Molecular and Morpho-agronomic Characterization of NaN3-induced Common Bean Mutants

疊氮化鈉誘變之菜豆突變系分子與形態農藝特性研究

王晞華(Hi-Hua Wang) ； 鄭统隆(Toong-Long Jeng) ； 陳宗禮(Tsung-Li Chen) ； 莊淑珍(Su-Jene Chuang) ； 魏夢麗(Meng-Li Wei) ； 宋濟民(Jih-Min Sung)

《作物、環境與生物資訊》 7卷4期 (2010/12) Pp. 221-232

https://doi.org/10.30061/CEB.201012.0001

摘要

本研究利用擴增增殖片段長度多型性及形態與農藝性狀之差異來探討菜豆品種花莢種及其34個疊氮化鈉誘變突變系之遺傳變異。所使用八個引子對在供試突變系共產生482個DNA片段，其中402個DNA片段為多形性片段。依據擴增增殖片段長度多型性資料所估算之Jaccard相似性係數介於0.469與0.839之間。群叢及主軸分析確認花莢種及其34個疊氮化鈉誘變突變系的確有明顯的分子層次變異。供試材料間也有明顯的形態與農藝性狀差異。此二種特性也有極顯著的相關性(r=0.713(上標 **))。因此，疊氮化鈉引起的誘變的確擴大遺傳變異，並可能產生新的菜豆品種。其中突變系SA-07，SA-08，SA-09，SA-14，SA-30及SA-31可做為育成新品種之材枓。

關鍵字

增增殖片段長度多型性 ； 花豆 ； 遺傳相似性 ； 形態與農藝性狀

並列摘要

In this study, amplified fragment length polymorphism (AFLP) and morpho-agronomic diversities in Hwachia, a common bean (Phaseolus vulgaris L.) variety, and its 34 NaN3-induced mutants (M6 generation) were investigated. Eight primer combinations generated 482 fragments from the tested materials, of which 402 fragments were polymorphic. The calculated Jaccard similarity coefficients based on AFLP data ranged from 0.469 to 0.839. Both clustering and principal coordinate analyses confirmed the presence of molecular diversities among Hwachia and its mutants. Morpho-agronomic variations were also observed among the tested mutants. The relations between Hwachia and its mutants based on AFLP data were correlated with those based on morpho-agronomic characteristics (r=-0.713(superscript **)). Thus, NaN3-induced mutagenesis indeed broadened the genetic variation, and possibly produced new varieties in the common bean. Mutants SA-07, SA-08, SA-09, SA-14, SA-30 and SA-31 are useful as sources for deriving new varieties.

並列關鍵字

AFLP ； Common bean ； Genetic similarity ； Morpho-agronomic trait