篩選適合落花生毛狀根生長之簡化培養基

Selection of Simplified Medium Suitable for Hairy Root Growth of Peanut

蔡杏枚(Hsing-Mei Tsai) ； 廖宇賡(Yue-Ken Liao)

《作物、環境與生物資訊》 8卷4期 (2011/12) Pp. 242-253

https://doi.org/10.30061/CEB.201112.0004

摘要

In the present study, a cost effective simplified medium for culturing hairy roots of peanut (Arachis hypogaea L.) was developed. The Agrobacterium rhizogenes BCRC 15010 (ATCC 15834) was first applied to infect leaf explants of in vitro grown peanut plants (Tainan Selected 9; TNS 9), from which the hairy roots were induced as experimental materials. Hairy root cultures were then incubated on media made of instant soluble inorganic fertilizers with designated concentrations. The Concentrations of better combinations were chosen for further investigation of the promotion effect of organic supplements. The results revealed that the best medium composition was that adding Hyponex No. 1 fertilizer (H-1) as replacement of inorganic salts and potato extract (PE) as organic supplement. The growth of hairy roots under this simplified medium exhibited similar fresh weight increase (p ＞ 0.05) as those grown under MS medium. The price for purchasing the additives for preparing the simplified medium is only 8% of the expenditure for those needed in preparation of MS medium. Consequently this medium can be considered a low cost formula for commercial use.

關鍵字

落花生 ； 毛狀根 ； 花寶1號 ； 馬鈴薯 ； 抽出物 ； 簡化培養基

並列摘要

In the present study, a cost effective simplified medium for culturing hairy roots of peanut (Arachis hypogaea L.) was developed. The Agrobacterium rhizogenes BCRC 15010 (ATCC 15834) was first applied to infect leaf explants of in vitro grown peanut plants (Tainan Selected 9; TNS 9), from which the hairy roots were induced as experimental materials. Hairy root cultures were then incubated on media made of instant soluble inorganic fertilizers with designated concentrations. The Concentrations of better combinations were chosen for further investigation of the promotion effect of organic supplements. The results revealed that the best medium composition was that adding Hyponex No. 1 fertilizer (H-1) as replacement of inorganic salts and potato extract (PE) as organic supplement. The growth of hairy roots under this simplified medium exhibited similar fresh weight increase (p ＞ 0.05) as those grown under MS medium. The price for purchasing the additives for preparing the simplified medium is only 8% of the expenditure for those needed in preparation of MS medium. Consequently this medium can be considered a low cost formula for commercial use.

並列關鍵字

Arachis hypogaea ； Hairy root ； Hyponex 1 ； Potato extract ； Simplified medium