Sub-health status was a sensitive physical state between health and illness. It may be turned featuring physiological function deterioration or in good health. The trends in attitudes toward life of people were without taking medicine, emphasizing self-healing cure and keeping away from cancer. Sub-health attracted our attention to explore it from three aspects: etiology, pathogenesis, clinical manifestations, prevention and treatment through traditional Chinese medicine constitution theory. For prevention of human life, this paper discussed to research the assessment and diagnosis of sub-health by Chinese medicine study or CAM therapy. Therefore, we could be in pursuit of a better state of physical and mental human healthy care.