This research explores the person-organization fit (p-o fit) and the influence of emotional intelligence variance on people's job rotation preference. The research population is com-prised of executive staff members in 20 private colleges in central Taiwan. A total of 300 questionnaires were distributed with 267 forms returned, of which, 259 were valid. The re-search result reveals that gender, age, marital status, annual salary and frequency of job rota-tion had no obvious influence on the job rotation preference. The job rotation preference is highest in people with junior college degrees and bachelor's degrees. The higher the p-o fit, the higher the job rotation preference. In addition, emotions have a positive influence on the job rotation preference and the p-o fit becomes more apparent in staff with a high degree of emotional intelligence compared to one with a low degree of emotional intelligence. There is a more significant and positive influence on the job rotation preference and p-o fit of a staff with great emotional intelligence than one with poor emotional intelligence. Based on the above findings, the researcher suggests several applications and recommends follow-up stud-ies.