Effect of Cultured Cumulus Cells as Donor Cells on Reconstructed Porcine Oocytes

以不同來源之卵丘細胞為供核細胞對重組豬卵母細胞之影響

林育安(Yu-An Lin) ； 蔡宏斌(Hong-Bing Tsai) ； 廖明輝(Ming-Huei Liao) ； 陳銘正(Ming-Cheng Chen)

《國立宜蘭大學工程學刊》 6期 (2010/03) Pp. 25-34

https://doi.org/10.6176/BCE.2010.06.13

摘要

The effects of cultured cumulus cells as donor cells on the genetically reconstructed porcine oocytes, matured in vitro, after nuclear transfer were studied. The cultured cumulus cells (F4) and the freshly acquaired cumulus cells (F0) were injected into the perivitelline space of the enucleated oocytes to obtain the genetically reconstructed oocytes. The oocytes were fused and activated simultaneously. These oocytes were observed for the embryo development for 144 h. It was found that the activation rate and the one-pronuclear formation (1N) of the F4 group were significantly higher than the F0 group (p＜0.05). Also, the embryo development rate of the F4 group was significantly higher than the F0 group (p＜0.05). In addition, the cultured cumulus cells enhanced the genetically reconstructed oocytes to advance their development to such stage as morula. These effects were attributed a better synchronization of the donor cells with the recipient cells of the F4 group.

關鍵字

豬卵母細胞 ； 體外成熟 ； 卵丘細胞 ； 核移置 ； 胚發育

並列摘要

The effects of cultured cumulus cells as donor cells on the genetically reconstructed porcine oocytes, matured in vitro, after nuclear transfer were studied. The cultured cumulus cells (F4) and the freshly acquaired cumulus cells (F0) were injected into the perivitelline space of the enucleated oocytes to obtain the genetically reconstructed oocytes. The oocytes were fused and activated simultaneously. These oocytes were observed for the embryo development for 144 h. It was found that the activation rate and the one-pronuclear formation (1N) of the F4 group were significantly higher than the F0 group (p＜0.05). Also, the embryo development rate of the F4 group was significantly higher than the F0 group (p＜0.05). In addition, the cultured cumulus cells enhanced the genetically reconstructed oocytes to advance their development to such stage as morula. These effects were attributed a better synchronization of the donor cells with the recipient cells of the F4 group.

並列關鍵字

Porcine oocyte ； In vitro maturation ； Cumulus cells ； Nuclear transfer ； Embryo development