The purpose of this study was intended to explore the relationship between job characteristics and job satisfaction of the physical education teachers in universities and colleges. The questionnaire was used on this study and total 225 subjects were return. The data analysis has included descriptive analysis, t-test, one-way ANOVA, Pearson's correlation analysis, regression. The findings were as follows: The background variables of the physical education teachers have significantly and partially different on the job characteristic and job satisfaction. 2. There is significantly positively correlation between job characteristic and job satisfaction of physical education teachers in universities and colleges. 3. The job characteristic significantly predict 25.5 % of variance of job satisfaction. According to the above research finding, this study provided some suggestions for physical education teachers and the future studies.