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靈芝固態發酵產物之多醣和抗氧化活性分析

Analyses of Polysaccharide and Antioxidant activities from Ganoderma lucidum Solid-state Fermentative Products

摘要


靈芝為中國傳統的藥用真菌,其中以多醣體為靈芝中最具有藥療活性之物質,其具有降血糖、消炎和抗腫瘤作用。本研究為先利用小麥和大豆穀類基質、培養天數及水分含量等因素,進行靈芝固態發酵。以酚硫法分析靈芝固態發酵產物的多醣含量;再將小麥和大豆基質之固態發酵產物進行抗氧化分析,結果顯示,以小麥基質之固態發酵產物,水分和基質的比例控制在0.6:1發酵一星期具有較高濃度的多醣。靈芝固態發酵以小麥和大豆作為培養基,結果顯示大豆培養基的靈芝固態發酵產物具有較佳的抗氧化特性,包括清除DPPH自由基能力、螫合亞鐵離子能力和還原力。

關鍵字

靈芝 固態發酵 多醣 抗氧化

並列摘要


Ganoderma lucidum is the Chinese traditional medicinal mushroom-like fungus. The polysaccharide is one of valuable and bioactive Ganoderma lucidum metabolites, and it has hypoglycemic activity, reducing inflammation and anti-tumor activity. The Ganoderma lucidum solid-state fermentation operated at different media (wheat or soybean), the ratio of water to medium, and fermentation were studied. The polysaccharide contents in fermentative products were analyzed by phenol-sulfuric acid method. The abilities of scavenging DPPH free radicals, reducing power, and chelating effect of ferrous ion were evaluated as the antioxidant activities of the hot water extracts from Ganoderma lucidum solid-state fermentative products. The results showed that the one week wheat fermentative products had higher polysaccharide content and the soybean fermentative products had better antioxidant activities.

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