大花咸豐草不同部位之抗氧化性

Antioxidant Properties of Different Portions of B. pilosa var. radiata

江淑華(Shu-Hua Chiang) ； 陳志瑋(Chin-Wei Chen) ； 王秀育(Shiu-Yu Wang) ； 賴潔賢(Chieh-Hsien Lai) ； 張基郁(Chi-Yue Chang)

《科學與工程技術期刊》 4卷3期 (2008/09) Pp. 1-7

https://doi.org/10.7117/JSET.200809.0001

摘要

本研究以大花咸豐草(B. pilosa var. radiate)爲材料，經烘箱乾燥後，將各部位分別研磨成粉狀，經由甲醇萃取並以減壓濃縮處理後，取其濃縮物分別進行花、莖及葉三部位之抗氧化性測定。抗氧化性測定項目，包括還原力、亞鐵離子螯合能力及DPPH (α, α-diphenyl-β-picrylhydrazyl)自由基清除能力等三種，並與EDTA(ethylenediamine tetraacetic acid)、BHA(butylated hydroxyanisole)及α-生育醇之抗氧化性做比較。結果發現，還原力方面，大花咸豐草各部位的甲醇萃取物都有強的還原力，其順序依序爲花＞葉＞莖。尤其花的萃取物在樣品重對溶劑體積比爲1.28 mg/mL時，即與α-生育醇及BHA相當。在亞鐵離子螯合能力方面，大花咸豐草各部位的甲醇萃取物其螯合能力並不顯著。在DPPH自由基清除能力方面，其三部位之自由基清除能力，以花與葉的萃取物效果最佳，分別在樣品重對溶劑體積比爲1.28mg/mL與0.37 mg/mL時，即與BHA及α-生育醇相當；莖的萃取物在樣品重對溶劑體積比爲6.38 mg/mL時亦有與BHA及α-生育醇相當之自由基清除能力。

關鍵字

大花咸豐草 ； 抗氧化性 ； 還原力 ； 亞鐵離子螯合能力 ； DPPH自由基清除能力

並列摘要

The flower, leaf, and stem of B. pilosa var. radiata were used as materials in this study. After oven-drying, these three portions were extracted with methanol, and then the solvent of the extracts was evaporated out under reduced pressure. The antioxidant properties of the extracts, including the reducing power, ferrous ion chelating power, α, α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity were measured and compared with those of alpha-tocopherol and butylated hydroxyanisole (BHA). Data showed that the methanol extracts from these three portions all had high reducing power, the sequence of these three portions was flower＞leaf＞stem, which were as high as those of alpha-tocopherol and BHA when the sample weight/solvent volume ratio was at 1.28 mg/mL. As for the results for the ferrous ion chelating power, the methanolic extracts showed low ferrous ion chelating power in all three portions. The DPPH radical scavenging activity results showed that the methanolic extracts from these three portions exhibited high values, especially flower and leaf, which were as high as those of alpha-tocopherol and BHA when the sample weight/solvent volume ratio of the flower portion was 1.28 mg/mL, and the sample weight/solvent volume ratio of leaf portion was 0.37 mg/mL. When the sample weight/solvent volume ratio of stem portion was 6.38 mg/mL, it also showed the DPPH radical scavenging activity which was as high as those of alpha-tocopherol and BHA.

並列關鍵字

B. pilosa var. radiate ； antioxidant properties ； reducing power ； ferrous ion chelating ability ； DPPH radical scavenging activity