探討奈米膠體金對盤尼西林醯胺酵素之轉化效率影響

Investigation of Nano-colloidal Gold to the Bioconversion Efficiency of Penicillin G Acylase

陳志義(Chih-I Chen) ； 陳品妡(Pin-Hsin Chen)

《修平學報》 27期 (2013/09) Pp. 1-10

摘要

本研究應用奈米膠體金（Colloidal Gold，CG）與盤尼醯林醯胺酵素（penicillin G acylase，PGA），進行PG生化反應生成6-APA之轉化效率影響研究。首先製作不同粒徑之CG，利用其添加量、pH值和溫度，探討上述因素對6-APA反應轉化效率之影響。實驗結果證實，添加CG可提高PGA對反應生成6-APA之轉化效率。其最佳添加條件為：PGA加0.05ml CG溶液（D=46.5nm），在pH8、37℃條件下，添加CG之轉化效率較未添加CG的增加2.13倍。另外，實驗發現CG效應為降低PG反應的活化能（降低11.7%），使得生成6-APA之轉化效率提高。將CG經重複使用7次，仍維持生成6-APA之轉化效率效果。

關鍵字

膠體金 ； 盤尼醯林醯胺酵素 ； 奈米 ； 活化能

並列摘要

In this study, we focus on the effect of nano-colloidal gold (CG) on the conversion efficiency of PG to 6-APA. Those influential factors under study are CG particle size, addition amount, pH value and solution temperature. From the experimental result, it shows that conversion efficiency could be increased 2.13 folds with the help of CG. The optimal conditions are as follows: adding 0.05 ml of CG (D = 46.5 nm) to pH 8 PGA solution at 37 ℃. It was also found that CG could lowering down about 11.7% activation energy of the PG hydrolysis reaction, which made the conversion efficiency to be higher than ever. And CG would be reusable, which could maintain the conversion efficiency of 6-APA nearly the same even repeated used 7 times.

並列關鍵字

colloidal gold (CG) ； Penicillin G Acylase (PGA) ； nano ； the activation energy

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