Basidiomatal Formation in Antrodia cinnamomea from the Perspective of Gene Expression

為了釐清樟芝不同型態之分化過程，我們分別自液態培養的樟芝菌絲體及野生之樟芝子實體建構二個互補股DNA的基因資料庫。經由單向之解序結果，分別穫得了821及993個高品質之表現序列標籤，續由BLASTX的分析結果顯示，與現有已知序列的資料庫之相似度僅有32.5%-33.7%（以期望值≤10^(-10)為界）。不論是菌絲體或是子實體的資料庫，其中，屬於代謝相關的基因數目最多，接著是屬於與蛋白質的表現時期和蛋白質生合成有關之基因數目次多，而兩組互補股DNA的基因資料庫之間則以「細胞時期、細胞週期及DNA生成」一組所表現的基因差異最大，接著是與代謝有關之基因數目次多。藉由本研究所建構的序列資訊，將有助於克服樟芝所面臨難以促進子實體形成之難題，並可提供未來調控代謝物研究時之重要參考訊息。

關鍵字

樟芝；基因表現標籤；藥用真菌；型態分化

並列摘要

In order to understand the phenomenon of morphological differentiation in medicinal fungus Antrodia cinnamomea, two complementary DNA (cDNA) libraries were constructed from the liquid-cultured mycelia (AM) and natural basidiomes (AT) produced from the infested wood. Using single-passed sequencing of cDNA clones, 821 and 993 high-quality expressed sequence tags (ESTs) were generated from the liquid-cultured mycelial and wild basidiomatal cDNA libraries, respectively. The results from BLASTX search revealed that only 32.5% to 33.7% of ESTs showed significant similarity to protein sequences in public databases (E values ≤10^(-10). The cDNAs encoding genes related to metabolism were found to be most abundant, followed by genes involved in protein fate and protein synthesis in each category. Genes related to the ”Cell fate, cell cycle, and DNA processing” category showed the greatest difference between the liquid-cultured mycelial and wild basidiomatal cDNA libraries, followed by genes involved in metabolism. The results from this study have provided valuable sequence information, which may lead to improved production of basidiomes of A. cinnamomea and regulation of metabolites in the future.

並列關鍵字

Antrodia cinnamomea ； Expressed sequence tags ； Medicinal fungus ； Morphological differentiation

國際替代計量

Basidiomatal Formation in Antrodia cinnamomea from the Perspective of Gene Expression

全文下載

主題瀏覽