- 期刊
Isolation, Characterization and phosphate-starvation Inducible Expression of Potential Brassica napus PURPLE ACID PHOSPHATASE 17 (BnPAP17) Gene Family
甘藍型油菜潛在的紫色酸性磷酸酶17(BnPAP17)基因家族的克隆、分析和磷饑餓誘導表達
摘要
本研究克隆了甘藍型油菜紫色酸性磷酸酶PAP17 (BnPAP17)基因家族的3個成員。BnPAP17-1、BnPAP17-2和BnPAP17-3的全長cDNA分別為1277、1356和1349bp，基因組序列分別為1466、1594和1598bp。推定的BnPAP17-1、BnPAP17-2和BnPAP17-3蛋白分別包含337、333和333個氨基酸殘基，它們均為分泌性低分子量(LMW)PAP，包含金屬磷(metallophos)結構域、5個保守性基序和7個金屬離子結合殘基。BnPAP17-2與BnPAP17-3高度相似，而它們與BnPAP17-1差異較大。Southern雜交進一步證實這3個成員構成了整個BnPAP17家族。RT-PCR分析表明BnPAP17家族成員主要在生殖器官中表達，尤以蕾中表達量最高。在營養器官中，BnPAP17-2和BnPAP17-3在根、下胚軸和莖中有一定程度的表達，而BnPAP17-1只在根中表達。在苗期，它們均受磷饑餓強烈誘導，而恢復供磷後表達量回復到誘導前水準，表明這些成員在植株的生殖發育和對磷缺乏的適應性中發揮重要作用。
並列摘要
Three members of a Brassica napus PURPLE ACID PHOSPHATASE 17 (BnPAP17) gene family were isolated. The full-length cDNAs of BnPAP17-1, BnPAP17-2 and BnPAP17-3 are 1277, 1356 and 1349 bp, with corresponding genomic sequences of 1466, 1594 and 1598 bp, respectively. The deduced 337-aa BnPAP17-1, 333-aa BnPAP17-2 and 333-aa BnPAP17-3 proteins are all secretary low molecular weight (LMW) PAPs, containing a metallophos domain, 5-block conserved motifs and 7 metal-ligating residues. BnPAP17-2 and BnPAP17-3 are highly similar to each other, but distinct from BnPAP17-1. Southern analysis suggests that these three genes comprise the entire BnPAP17 gene family. They are all mainly transcribed in reproductive organs especially in bud. In vegetative organs, BnPAP17-2 and BnPAP17-3 are expressed in root, hypocotyls and stem, while BnPAP17-1 expression is limited to root. In seedlings, these genes are all strongly induced by phosphate-starvation, and return to basal levels after phosphate resupply. Thus they are suggested to play important roles in reproductive development and adaptation to phosphorus deficiency.
延伸閱讀
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